The large-scale farming of Nannochloropsis spp. has long been subject to high temperature. Though salicylic acid is widely used to enhance the high temperature resistance of higher plants, related research in microalgae is still in its infancy. In this study, Nannochloropsis oceanica cells at 25 °C were transferred to high temperature (35 °C) for 96 h, during which period salicylic acid with various concentrations (0, 15, 25, 35, and 50 mg L⁻¹) were added into high temperature groups. The results indicated that high temperature could heavily inhibit the growth of N. oceanica , and result in various physiological and biochemical changes. The addition of exogenous salicylic acid at moderate concentration distinctly alleviated the inhibition of heat stress on N. oceanica . Moreover, further analysis indicated that exogenous salicylic acid could significantly increase the contents of soluble sugars, soluble proteins, total unsaturated fatty acids, and catalase activity, but result in the decrease of the nitrate reductase activity, the malondialdehyde content, and fatty acid profile. Our results also demonstrated that salicylic acid at a concentration of 15 mg L⁻¹ received the best growth performance of N. oceanica under 35 °C. This study investigates the thermal protection effect of salicylic acid on N. oceanica under high temperature, and can provide valuable information for the large-scale production of microalgae.

中文翻译：

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外源水杨酸在提高 拟南芥 耐高温性中的应用

Nannochloropsis spp的大规模养殖 。长期以来一直处于高温状态。尽管水杨酸被广泛用于增强高等植物的耐高温性，但有关微藻的相关研究仍处于起步阶段。在这项研究中，将 25°C的 拟南芥海洋 细胞转移至高温（35°C）96 h，在此期间，各种浓度（0、15、25、35和50 mg L ^-1）的水杨酸加入高温组。结果表明，高温可以严重抑制 海洋猪笼草的生长 。 ，并导致各种生理和生化变化。外源水杨酸的中度浓度明显减轻了 海洋烟草 对热胁迫的抑制作用 。此外，进一步的分析表明，外源水杨酸可以显着增加可溶性糖，可溶性蛋白质，总不饱和脂肪酸和过氧化氢酶活性的含量，但导致硝酸还原酶活性，丙二醛含量和脂肪酸谱的降低。我们的结果还证明，在35°C下，浓度为15 mg L ^-1的水杨酸具有最佳的 海洋猪笼草 生长性能 。本研究探讨了水杨酸对人体的热保护作用 N. oceanica 在高温下，可以为大规模生产微藻提供有价值的信息。