As two prominent examples of intracellular single-domain antibodies or antibody mimetics derived from synthetic protein scaffolds, monobodies and nanobodies are gaining wide applications in cell biology, structural biology, synthetic immunology, and theranostics. We introduce herein a generally-applicable method to engineer light-controllable monobodies and nanobodies, designated as moonbody and sunbody, respectively. These engineered antibody-like modular domains enable rapid and reversible antibody-antigen recognition by utilizing light. By paralleled insertion of two LOV2 modules into a single sunbody and the use of bivalent sunbodies, we substantially enhance the range of dynamic changes of photo-switchable sunbodies. Furthermore, we demonstrate the use of moonbodies or sunbodies to precisely control protein degradation, gene transcription, and base editing by harnessing the power of light.

中文翻译：

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具有光敏开关的智能抗体模拟物的设计

作为衍生自合成蛋白支架的细胞内单域抗体或抗体模拟物的两个突出实例，单克隆抗体和纳米抗体在细胞生物学，结构生物学，合成免疫学和治疗学中得到了广泛的应用。我们在本文中介绍了一种普遍适用的方法来工程化可控光的单体和纳米抗体，分别命名为月体和日体。这些工程化的抗体样模块化结构域通过利用光实现了快速且可逆的抗体-抗原识别。通过将两个LOV2模块并行插入一个单一的太阳体中并使用二价太阳体，我们大大增强了可光转换太阳体的动态变化范围。此外，我们演示了使用月体或日体来精确控制蛋白质降解，基因转录，