Abstract Context Fruit of Avicennia marina (Forsk.) Vierh. (Acanthaceae) is used as a Chinese herb. Studies have found that it contains marinoid J, a novel phenylethanoid glycoside (PG) compound, but its neuroprotective functions are largely unknown. Objective This study evaluated the effects of marinoid J on vascular dementia (VD) and determined its potential mechanisms of action. Materials and methods The VD model was established by the ligation of the bilateral common carotid artery in Sprague–Dawley rats, who received daily intragastrically administration of saline, marinoid J (125 or 500 mg/kg body weight/d), or oxiracetam (250 mg/kg body weight/d) for 14 days (20 rats in each group). The Morris water maze (MWM) was used to evaluate cognitive performance. The hippocampus was subjected to histological and proteomic analyses. Results Marinoid J shortened the escape latency of VD rats (31.07 ± 3.74 s, p < 0.05). It also decreased malondialdehyde (MDA) (27.53%) and nitric oxide (NO) (20.41%) while increasing superoxide dismutase (SOD) (11.26%) and glutathione peroxidase (GSH-Px) (20.38%) content in hippocampus tissues. Proteomic analysis revealed 45 differentially expressed proteins (DEPs) in marinoid J-treated VD rats, which included angiotensin-converting enzyme (ACE), keratin 18 (KRT18), cluster of differentiation 34 (CD34), and synaptotagmin II (SYT2). Conclusions Marinoid J played a role in protecting hippocampal neurons by regulating a set of proteins that influence oxidative stress and apoptosis, this effect may thereby alleviate the symptoms of VD rats. Thus, pharmacological manipulation of marinoid J may offer a novel opportunity for VD treatment.

中文翻译：

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Marinoid J，一种来自 Avicennia marina 果实的苯基糖苷，可改善大鼠血管性痴呆的认知障碍：定量 iTRAQ 蛋白质组学研究

Avicennia marina (Forsk.) Vierh 的抽象背景果实。(Acanthaceae) 被用作中草药。研究发现它含有 marinoid J，一种新型的苯乙醇苷 (PG) 化合物，但其神经保护功能在很大程度上是未知的。目的 本研究评估了海鱼 J 对血管性痴呆 (VD) 的影响并确定其潜在的作用机制。材料和方法 VD 模型是通过结扎 Sprague-Dawley 大鼠的双侧颈总动脉建立的，这些大鼠每天接受盐水、marinoid J（125 或 500 mg/kg 体重/天）或奥拉西坦（250 mg/kg 体重/天），持续 14 天（每组 20 只大鼠）。Morris水迷宫（MWM）用于评估认知能力。对海马体进行组织学和蛋白质组学分析。结果 Marinoid J 缩短了 VD 大鼠的逃逸潜伏期（31.07 ± 3.74 s，p < 0.05）。它还降低了丙二醛 (MDA) (27.53%) 和一氧化氮 (NO) (20.41%)，同时增加了海马组织中超氧化物歧化酶 (SOD) (11.26%) 和谷胱甘肽过氧化物酶 (GSH-Px) (20.38%) 的含量。蛋白质组学分析揭示了海胆类 J 治疗的 VD 大鼠中有 45 种差异表达蛋白 (DEP)，其中包括血管紧张素转换酶 (ACE)、角蛋白 18 (KRT18)、分化簇 34 (CD34) 和突触结合蛋白 II (SYT2)。结论 Marinoid J通过调节一组影响氧化应激和细胞凋亡的蛋白质发挥保护海马神经元的作用，该作用可减轻VD大鼠的症状。因此，marinoid J 的药理学操作可能为 VD 治疗提供新的机会。