miRNA 146b mediates the regulation of nucleolar size and activity in polyploid megakaryocytes,Biology of the Cell

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miRNA 146b mediates the regulation of nucleolar size and activity in polyploid megakaryocytes
Biology of the Cell ( IF 2.4 ) Pub Date : 2020-12-28 , DOI: 10.1111/boc.202000022
Saran Chattopadhyaya ₁ , Subrata Banerjee ₂

Affiliation

BACKGROUND INFORMATION Megakaryocytes (MKs) follow a unique cell cycle duplication process, called endomitosis, resulting in polyploidization of cells. It is hypothesized that polyploidy, as well as an increment in cytoplasm volume, allow more efficient platelets generation from MKs. Although polyploidy leads to an increase in the DNA amount, which impacts gene expression, little is known about ribosomal biogenesis in these polylobulated polyploid cells. RESULTS The nucleolus acts as a hub for ribosomal biogenesis, which in turn governs the protein synthesis rate of the cells. We therefore estimated the size and activity of the nucleolus in polyploid cells during megakaryopoiesis in vitro. Polyploid megakaryocytic cell lines and in vitro cultured MKs, which were obtained from human cord blood-derived CD 34+ cells, revealed that miRNA 146b regulated the activity of nucleolar and coiled-body phosphoprotein 1 (NOLC1), which plays an integral role in determining nucleolar size and activity. Additionally, miRNA-146b was upregulated during endomitosis and was found to promote megakaryopoiesis. CONCLUSION We propose that miRNA 146b regulates not only nucleolar size and activity, but also megakaryopoiesis. SIGNIFICANCE This study highlights the importance of nucleolar activity and miRNA in the progression of megakaryopoiesis and thrombopoiesis. This article is protected by copyright. All rights reserved.

中文翻译：

miRNA 146b 介导多倍体巨核细胞核仁大小和活性的调节

背景信息巨核细胞 (MK) 遵循独特的细胞周期复制过程，称为内有丝分裂，导致细胞多倍化。据推测，多倍体以及细胞质体积的增加允许更有效地从 MK 生成血小板。尽管多倍体导致 DNA 量增加，从而影响基因表达，但对这些多分叶多倍体细胞中的核糖体生物发生知之甚少。结果核仁作为核糖体生物发生的枢纽，进而控制细胞的蛋白质合成速率。因此，我们估计了体外巨核细胞生成过程中多倍体细胞中核仁的大小和活性。多倍体巨核细胞系和体外培养的 MKs，从人脐带血来源的 CD 34+ 细胞中获得，揭示miRNA 146b调节核仁和卷曲体磷蛋白1（NOLC1）的活性，其在确定核仁大小和活性中起着不可或缺的作用。此外，miRNA-146b 在内丝分裂过程中被上调，并被发现促进巨核细胞生成。结论我们提出 miRNA 146b 不仅调节核仁大小和活性，还调节巨核细胞生成。意义本研究强调了核仁活性和 miRNA 在巨核细胞生成和血小板生成进程中的重要性。本文受版权保护。版权所有。结论我们提出 miRNA 146b 不仅调节核仁大小和活性，而且调节巨核细胞生成。意义本研究强调了核仁活性和 miRNA 在巨核细胞生成和血小板生成进程中的重要性。本文受版权保护。版权所有。结论我们提出 miRNA 146b 不仅调节核仁大小和活性，而且调节巨核细胞生成。意义本研究强调了核仁活性和 miRNA 在巨核细胞生成和血小板生成进程中的重要性。本文受版权保护。版权所有。

更新日期：2020-12-28

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