In this study, the anti-cancer compound was extracted from Chrysanthemum morifolium (C. morifolium) by soxhlet apparatus method. The available anti-cancer compounds of Chrysanthemum morifolium extract were clearly detected by LC-MS analysis based on the retention time, percentages of height and occupied area. The anti-cancer compounds were separately screened from mixed compounds by NIST Wiley library interpretation. Further, the silver nanoparticle (Ag NPs) was synthesized from C. morifolium extract, and their surface plasmon resonance effect of the extract was scanned by UV–vis spectroscopy at 410 nm. Morphological identification of size and shape of the Ag NPs was confirmed by scanning electron microscope and transmission electron microscope images. Further, the anti-cancer effect of synthesized Ag NPs was exhibited more cytotoxicity effect at the concentration of 200 µg/mL. Furthermore, the morphological damages of A549 cells were clearly confirmed by fluorescence microscope using AO/EB fluorescence stain. Finally, the results were suggested that the biosynthesized Ag NPs has potential anti-cancer agent against A549 lung cancer cells.

在这项研究中，抗癌化合物从萃取菊花（C.菊花）通过索格利特装置的方法。根据保留时间，高度百分比和占据面积，通过LC-MS分析清楚地检测了菊花提取物的可用抗癌化合物。通过NIST Wiley文库解释从混合的化合物中分别筛选出抗癌化合物。此外，由mo霉（C. morifolium）合成了银纳米颗粒（Ag NPs）提取物，并通过紫外可见光谱在410 nm扫描提取物的表面等离子体共振效应。通过扫描电子显微镜和透射电子显微镜图像证实了Ag NP的尺寸和形状的形态学鉴定。此外，在200 µg / mL的浓度下，合成的Ag NPs的抗癌作用表现出更大的细胞毒性作用。此外，通过使用AO / EB荧光染料的荧光显微镜清楚地证实了A549细胞的形态损伤。最后，结果表明生物合成的银纳米颗粒具有潜在的抗A549肺癌细胞的抗癌药。