MicroRNAs serve as potential biomarkers in various pathological models, and are stable and detectable in biofluids. We investigated the urinary microRNA expression profile in a gentamicin-induced acute kidney injury canine model using RNA sequencing. A total of 234 differentially expressed microRNAs were screened after 12 consecutive days of gentamicin administration (P < 0.05). Six candidate microRNAs (miR-15b, -15b-3p, -16, -30a, -30a-3p, and -30c-2-3p) were selected according to a set criterion, and validated by real-time quantitative PCR. The diagnostic values of these six candidate microRNAs were better than the traditional serum biomarkers (all P < 0.05). Further, using receiver operating characteristic curve analysis, we found that miR-15b and -15b-3p were superior to urinary kidney injury molecule-1 (both P < 0.05). Moreover, miR-15b and -30a levels in the urine samples significantly correlated with their respective levels in the kidney tissue samples (r=0.512 and 0.505, respectively, both P < 0.05). Our data concluded that miR-15b and -30a may be promising biomarkers for renal toxicity.

中文翻译：

---

尿液 microRNAs miR-15b 和 miR-30a 作为庆大霉素诱导的急性肾损伤的新型非侵入性生物标志物

MicroRNAs 在各种病理模型中作为潜在的生物标志物，在生物体液中是稳定和可检测的。我们使用 RNA 测序研究了庆大霉素诱导的急性肾损伤犬模型中的尿 microRNA 表达谱。连续 12 天庆大霉素给药后，共筛选出 234 个差异表达的 microRNA（P < 0.05）。根据设定的标准选择了六个候选 microRNA（miR-15b、-15b-3p、-16、-30a、-30a-3p 和 -30c-2-3p），并通过实时定量 PCR 进行验证。这6个候选microRNA的诊断价值优于传统的血清生物标志物（均P < 0.05）。此外，使用受试者工作特征曲线分析，我们发现 miR-15b 和-15b-3p 优于尿肾损伤分子-1（均 P < 0.05）。而且，尿液样本中的 miR-15b 和 -30a 水平与其各自在肾组织样本中的水平显着相关（r 分别为 0.512 和 0.505，均 P < 0.05）。我们的数据得出结论，miR-15b 和-30a 可能是肾毒性的有希望的生物标志物。