Abstract The present work analyzed the antibacterial, cytotoxic, and antidiabetic activities of endophytic Diaporthe eres (SPEF004). The profiling of bioactive molecules was determined using GC-MS analysis and these molecules were docked with breast cancer-related protein (HSP90). Among the extracts, FEAE (Fungal ethyl acetate extract) showed zone of inhibition in the range of 9.06 to 27.5 mm against Staphylococcus. aureus, Escherichia coli; Salmonella enterica, Bacillus cereus, and Listeria monocytogenes. The FEAE showed DPPH (61.53 ± 1.47 %), ABTS (85.62 ± 2.48 %) scavenging property, and inhibitory activity of 41.11 ± 1.52 % for α amylase and 13.28 ± 0.94 % for α glucosidase at the concentration of 100 µg/mL. Interestingly, FEAE was found to be not toxic to mouse fibroblast (NIH3T3) cells, but, the toxic to human breast cancer cell line (MDA MB231) through the damaging nucleus at an IC50 value of 27.5 ± 0.25 µg/mL. Furthermore, pyrrolidine-5-one, 2-[3-hydroxypropyl] derived from Diaporthe sp., exhibited a strong binding affinity score (-5.4 kcal/mol) with HSP90 through the interaction of hydrogen bonds, and it is expected to regulate the apoptosis in breast cancer cells. This work proved the bioactivities of the novel compounds identified from Diaporthe sp. (SPEF004) for the possible development of pharmaceutically viable agents.

中文翻译：

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本地内生真菌 (Diaporthe sp.) 的分子鉴定、挥发性代谢物分析和生物活性

摘要 目前的工作分析了内生 Diaportheres (SPEF004) 的抗菌、细胞毒性和抗糖尿病活性。使用 GC-MS 分析确定生物活性分子的谱，并将这些分子与乳腺癌相关蛋白 (HSP90) 对接。在提取物中，FEAE（真菌乙酸乙酯提取物）显示出对葡萄球菌的抑制范围为 9.06 至 27.5 毫米。金黄色葡萄球菌，大肠杆菌；沙门氏菌、蜡样芽孢杆菌和单核细胞增生李斯特菌。FEAE 显示 DPPH (61.53 ± 1.47 %)、ABTS (85.62 ± 2.48 %) 清除特性，α 淀粉酶的抑制活性为 41.11 ± 1.52 %，α 葡萄糖苷酶的抑制活性为 13.28 ± 0.94 %，μg/mL。有趣的是，FEAE 被发现对小鼠成纤维细胞 (NIH3T3) 没有毒性，但是，通过破坏细胞核对人乳腺癌细胞系 (MDA MB231) 产生毒性，IC50 值为 27.5 ± 0.25 µg/mL。此外，源自 Diaporthe sp. 的 pyrrolidine-5-one, 2-[3-hydroxypropyl] 通过氢键的相互作用表现出与 HSP90 的强结合亲和力评分（-5.4 kcal/mol），并有望调节乳腺癌细胞凋亡。这项工作证明了从 Diaporthe sp. 中鉴定的新化合物的生物活性。(SPEF004) 用于可能开发药学上可行的药物。有望调控乳腺癌细胞的凋亡。这项工作证明了从 Diaporthe sp. 鉴定的新化合物的生物活性。(SPEF004) 用于可能开发药学上可行的药物。有望调控乳腺癌细胞的凋亡。这项工作证明了从 Diaporthe sp. 鉴定的新化合物的生物活性。(SPEF004) 用于可能开发药学上可行的药物。