Despite sharing many of the traits that have allowed the genus Bacillus to gain recognition for its agricultural relevance, the genus Lysinibacillus is not as well-known and studied. The present study employs in vitro, in vivo, in planta, and in silico approaches to characterize Lysinibacillus fusiformis strain S4C11, isolated from the roots of an apple tree in northern Italy. The in vitro and in vivo assays demonstrated that strain S4C11 possesses an antifungal activity against different fungal pathogens, and is capable of interfering with the germination of Botrytis cinerea conidia, as well as of inhibiting its growth through the production of volatile organic molecules. In planta assays showed that the strain possesses the ability to promote plant growth, that is not host-specific, both in controlled conditions and in a commercial nursery. Biocontrol assays carried out against phytopathogenic viruses gave contrasting results, suggesting that the strain does not activate the host’s defense pathways. The in silico analyses were carried out by sequencing the genome of the strain through an innovative approach that combines Illumina and High-Definition Mapping methods, allowing the reconstruction of a main chromosome and two plasmids from strain S4C11. The analysis of the genes encoded by the genome contributed to the characterization of the strain, detecting genes related to the biocontrol effect detected in the experimental trials.

尽管共享了许多使芽孢杆菌属因其农业相关性而获得认可的特征，但赖氨酸杆菌属并不为人所知和研究。本研究采用体外、体内、植物和计算机模拟方法来表征从意大利北部一棵苹果树的根部分离出来的梭形赖氨酸杆菌菌株 S4C11。在体外和体内测定法证明了应变S4C11具有抗不同病原真菌的抗真菌活性，并且能够与萌发干扰灰霉病分生孢子，以及通过产生挥发性有机分子来抑制其生长。在植物试验中，该菌株具有促进植物生长的能力，这不是宿主特异性的，无论是在受控条件下还是在商业苗圃中。针对植物病原病毒进行的生物防治试验给出了截然不同的结果，表明该菌株不会激活宿主的防御途径。将在硅片通过结合 Illumina 和高清晰度图谱方法的创新方法对该菌株的基因组进行测序来进行分析，从而能够从菌株 S4C11 重建一条主染色体和两个质粒。对基因组编码的基因的分析有助于对菌株进行表征，检测与实验试验中检测到的生物防治效果相关的基因。