Porcine hemagglutinating encephalomyelitis virus (PHEV) is a member of the genus Betacoronavirus and is the etiologic agent of encephalomyelitis or vomiting and wasting disease in neonatal pigs. Although there are only a few epidemiological studies that document the seroprevalence of PHEV infection, there are reports of sporadic outbreaks, including recent documentation of an influenza-like respiratory disease associated with PHEV in the United States. To address this issue, we have developed a new indirect enzyme linked immunosorbent assay (ELISA) for use in sero-epidemiological research of PHEV infection. One hundred and fifty porcine serum samples that were determined as antibody-positive or antibody-negative in virus neutralization (VN) tests were used in conjunction with PHEV-specific antigen extracted from virus-infected FS-L3 cells using RBS buffer containing 0.2 % NP-40 to develop this assay. The ELISA showed a high sensitivity (95.35 %) and specificity (96.88 %) by receiver operating characteristic (ROC) analysis, with an area under the curve (AUC) of 0.996 attesting to its accuracy. Our results revealed a strong correlation between the results of the indirect ELISA and VN test (R = 0.850, P < 0.05), with near-perfect agreement (kappa value = 0.932). These results indicate that this new indirect ELISA might be useful for diagnosis and sero-epidemiological tracking of PHEV infection.

猪血凝性脑脊髓炎病毒（PHEV）是Betacoronavirus属的成员并且是新生猪脑脊髓炎或呕吐和消瘦疾病的病原体。尽管只有很少的流行病学研究记录了PHEV感染的血清流行，但是有零星爆发的报道，包括最近在美国有关与PHEV相关的流感样呼吸道疾病的报道。为解决此问题，我们开发了一种新的间接酶联免疫吸附测定（ELISA），可用于PHEV感染的血清流行病学研究。将150份在病毒中和（VN）测试中被确定为抗体阳性或抗体阴性的猪血清样品与使用含有0.2％NP的RBS缓冲液从病毒感染的FS-L3细胞中提取的PHEV特异性抗原结合使用-40开发此测定法。ELISA显示高灵敏度（95。接收器工作特性（ROC）分析得出35％的特异性和96.88％的特异性，曲线下面积（AUC）为0.996证明了其准确性。我们的结果显示间接ELISA结果与VN测试之间存在很强的相关性（R = 0.850，P <0.05），具有接近完美的一致性（kappa值= 0.932）。这些结果表明，这种新的间接ELISA可能对PHEV感染的诊断和血清流行病学追踪有用。