The populations of migrated small brown planthoppers (SBPHs) collected in Korea were reported to have higher insecticide resistance and percentages of viruliferous individuals than the populations of overwintering SBPHs. Therefore, the migrated SBPHs might survive after insecticide treatments and cause severe damage to rice plants. In this study, the changes in the biochemical properties of resistant SBPHs with temperature changes were investigated. The activities of detoxifying enzymes known to be involved in insecticide resistance showed no correlation with temperature, but the resistant strains had consistently higher detoxifying enzyme activities than the susceptible strains. Interestingly, the amount of reactive oxygen species (ROS) increased with temperature in all strains.

Chlorantraniliprole-resistant strains of Drosophila melanogaster were examined to investigate whether the phenomena observed in the resistant SBPHs were conserved in other insects with resistance to an insecticide with different modes of action. Similar to the resistant SBPHs, the resistant Drosophila also exhibited increased amounts of ROS and detoxifying enzyme activity compared to the control Drosophila. Also in the resistant Drosophila, only the ROS showed a temperature-dependent increase.

Taken together, in addition to the involvement of increased activities of detoxifying enzymes, the resistant insects also had a temperature-dependent significant increase in ROS. Thus, the development of tools to induce ROS toxicity could lead to the development of new control methods to eliminate resistant insect strains.

据报道，在韩国收集的移栽的小型褐飞虱种群比越冬的SBPH种群具有更高的杀虫抗性和有毒个体百分比。因此，迁移的SBPHs在经过杀虫剂处理后可能会存活下来，并严重危害水稻。在这项研究中，研究了抗性SBPHs的生化特性随温度的变化。已知与杀虫剂抗性有关的解毒酶的活性与温度没有关系，但是抗性菌株始终比易感菌株具有更高的解毒酶活性。有趣的是，在所有菌株中，活性氧（ROS）的数量均随温度增加而增加。

检查了对果蝇具有抗氯性的菌株，以研究在抗性SBPHs中观察到的现象是否在其他对不同作用方式的杀虫剂具有抗性的昆虫中得以保留。与抗性果蝇相似，与对照果蝇相比，抗性果蝇还表现出增加的ROS含量和解毒酶活性。同样在抗性果蝇中，仅ROS显示出温度依赖性的升高。

两者合计，除涉及增加解毒酶的活性外，抗性昆虫的ROS也随温度而显着增加。因此，诱导ROS毒性的工具的开发可能会导致消除有害抗性菌株的新控制方法的开发。