Fluorescent probes with high sensitivity, selectivity, and fast response are urgently required for fluorescent imaging. The introduction of silicon atom into coumarin skeleton have resolved abrupt and instant increment in fluorescent quantum yield (from 0.002 into 0.93) when the 3-azide was reduced to 3-amine. The hydrogen sulfide triggered reduction process has ultrafast kinetic and reached equilibrium within 25 s. A group of silicon substituted coumarins SiC-Azs were designed for sensitively monitoring cellular hydrogen sulfide in vivo. The probe SiC-Az2 can be highly selective and sensitive to monitor endogenous and exogenous hydrogen sulfide in Hela cells and zebrafish.

荧光成像迫切需要具有高灵敏度，选择性和快速响应的荧光探针。当3-叠氮化物还原为3-胺时，将硅原子引入香豆素骨架已解决了荧光量子产率的突然和瞬时增加（从0.002变为0.93）。硫化氢触发的还原过程具有超快的动力学，并在25 s内达到平衡。设计了一组硅取代的香豆素SiC-Az，用于敏感地监测体内的细胞硫化氢。探针SiC-Az2具有很高的选择性和敏感性，可监测Hela细胞和斑马鱼中的内源性和外源性硫化氢。