Generally, impaired bones heal by bone repair and bone regeneration. These two processes are necessary during the healing period of dental implant. Vasculature plays a crucial role in bone healing because bones are highly vascularized tissue. Osteogenesis and angiogenesis are highly coupled processes and can be regulated by Hippo-YAP signaling pathway. Recent studies have demonstrated Hippo-YAP pathway may be regulated by alpha calcitonin gene-related peptide. However, the regulatory effects of αCGRP-YAP pathway on angiogenesis and osteogenesis during bone healing around implants remain unclear. Four groups of mice were established: KO Group: αCGRP ^−/− mice; KO + αCGRP group: αCGRP ^−/− mice with αCGRP overexpressing lentiviral transfection; KO + YAP group: αCGRP ^−/− mice with YAP overexpressing lentiviral transfection; WT group: wildtype mice. After 14 or 28 days, animals were sacrificed and tested. Results showed αCGRP deficiency hampered osteogenesis and angiogenesis. In addition, the impaired bone healing can be rescued by overexpressing αCGRP and YAP in αCGRP ^−/− mice. In-vivo results indicate αCGRP-YAP pathway promotes angiogenesis and osteogenesis in bone healing, especially at the early stage. Taken together, present study demonstrated αCGRP up-regulate the expression of YAP, and down-stream genes to promote the osteogenesis and angiogenesis around the implants.

通常，受损的骨骼通过骨骼修复和骨骼再生来治愈。这两个过程在种植牙的愈合期间是必要的。血管系统在骨愈合中起着至关重要的作用，因为骨骼是高度血管化的组织。成骨和血管生成是高度耦合的过程，可以通过 Hippo-YAP 信号通路调节。最近的研究表明，Hippo-YAP 通路可能受 α 降钙素基因相关肽的调节。然而，αCGRP-YAP通路对种植体周围骨愈合过程中血管生成和成骨的调节作用仍不清楚。建立四组小鼠：KO组：αCGRP ^-/-小鼠；KO  +  αCGRP 组：αCGRP ^-/-αCGRP 过表达慢病毒转染的小鼠；KO  +  YAP组：过表达慢病毒转染的YAP的αCGRP ^-/-小鼠；WT组：野生型小鼠。14或28天后，处死动物并进行测试。结果显示αCGRP缺乏阻碍了成骨和血管生成。此外，通过在αCGRP ^-/-小鼠中过表达αCGRP和YAP可以挽救受损的骨愈合。体内结果表明 αCGRP-YAP 通路促进骨愈合中的血管生成和成骨，特别是在早期阶段。总之，本研究表明 αCGRP 上调 YAP 和下游基因的表达以促进植入物周围的成骨和血管生成。