Erysimum crepidifolium Rchb. is one of the few Brassicaceae species accumulating glucosinolates as well as cardenolides. This is possibly providing a selective advantage in evolution as both compounds are part of a chemical defense system. In order to study the biosynthesis of these compounds, a regeneration protocol for E. crepidifolium using in vitro shoot cultures derived from seeds has been developed. Murashige and Skoog (MS) culture medium supplemented with various combinations of cytokinins and auxins was used. MS medium containing NAA (naphthaleneacetic acid, 0.04 mg mL⁻¹) and BAP (6-benzylaminopurine, 0.2·10⁻² mg mL⁻¹) proved to be optimal for root formation. Plantlets developed well on modified MS medium without the use of phytohormones. About 80% of the plantlets rooted in vitro developed into intact plants after transfer to the greenhouse. Cardenolides (1.75 mg g⁻¹ dry weight (DW)) were detected in cultured shoots on solid DDV media while glucosinolates mainly accumulated in roots where 0.025 mg g⁻¹ FW were detected in shoots cultured on the same medium (DDV). The expression of two progesterone 5β-reductase and three Δ⁵-3β-hydroxysteroid dehydrogenase genes were measured in shoot cultures since the encoded enzymes are supposed to be involved in cardenolide biosynthesis. E. crepidifolium shoot cultures propagated on solid media meet the necessary requirements, i.e., clonal homogeneity, product accumulation, and gene expression, for a suitable model to study cardenolide but not glucosinolate biosynthesis.

糖芥crepidifolium Rchb 。是少数积累芥子油苷和芥子油苷的十字花科植物之一。由于这两种化合物都是化学防御系统的一部分，因此这可能在进化中提供选择优势。为了研究这些化合物的生物合成，已经开发了使用来源于种子的体外芽培养物对头孢大肠杆菌的再生方案。使用补充了细胞分裂素和生长素的各种组合的Murashige和Skoog（MS）培养基。包含NAA（萘乙酸，0.04 mg mL ^-1）和BAP（6-苄基氨基嘌呤，0.2·10 ^-2  mg mL ^-1）的MS培养基）被证明是最适合根形成的。在不使用植物激素的情况下，小苗在改良的MS培养基上发育良好。转移到温室中后，约有80％的体外生根小植株发育成完整的植株。在固态DDV培养基上培养的芽中检出了胡萝卜素（1.75 mg g ^-1干重（DW）），而芥子油苷则主要积累在根中，而在同一培养基（DDV）上培养的芽中检出了0.025 mg g ^-1 FW。两个表达孕酮5β还原酶和三个Δ ⁵ -3β羟类固醇脱氢酶在苗培养测定基因由于编码的酶都应该参与cardenolide生物合成。在固体培养基上繁殖的头孢鳞茎芽苗培养物满足必要的要求，即克隆同质性，产物积累和基因表达，这是适合于研究芥子油苷而不是芥子油苷生物合成的模型的要求。