Significance: Parallel phase-shifting digital holographic microscope (PPSDHM) is powerful for three-dimensional (3D) measurements of dynamic specimens. However, the PPSDHM reported previously was directly fixed on the optical bench and imposed difficulties case, thus it is required to modify the specification of the microscope or transport the microscope to another location. Aim: We present a modularized PPSDHM. We construct the proposed PPSDHM and demonstrate the 3D measurement capability of the PPSDHM. Approach: The PPSDHM was designed as an inverted microscope to record transparent objects and modularized by integrating the optical elements of the PPSDHM on an optical breadboard. To demonstrate the effectiveness of the PPSDHM, we recorded a 3D motion-picture of moving Volvoxes at 1000 frames / s and carried out 3D tracking of the Volvoxes. Results: The PPSDHM was practically realized and 3D images of objects were successfully reconstructed from holograms recorded with a single-shot exposure. The 3D trajectories of Volvoxes were obtained from the reconstructed images. Conclusions: We established a modularized PPSDHM that is capable of 3D image acquisition by integrating the optical elements of the PPSDHM on an optical breadboard. The recording capability of 3D motion-pictures of dynamic specimens was experimentally demonstrated by the PPSDHM.

中文翻译：

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基于平行相移数字全息成像的模块化显微镜，用于活体生物样本成像

意义：平行相移数字全息显微镜 (PPSDHM) 对于动态样本的三维 (3D) 测量非常强大。然而，之前报道的 PPSDHM 是直接固定在光具座上的，造成了困难，因此需要修改显微镜的规格或将显微镜运输到另一个位置。目标：我们提出了一个模块化的 PPSDHM。我们构建了提议的 PPSDHM 并展示了 PPSDHM 的 3D 测量能力。方法： PPSDHM 被设计为倒置显微镜，用于记录透明物体，并通过将 PPSDHM 的光学元件集成在光学面包板上进行模块化。为了证明 PPSDHM 的有效性，我们以 1000 帧/秒的速度记录了移动的沃尔沃克斯的 3D 电影，并对沃尔沃克斯进行了 3D 跟踪。结果： PPSDHM 被实际实现，并且从单次曝光记录的全息图成功重建了物体的 3D 图像。沃尔沃克斯的 3D 轨迹是从重建图像中获得的。结论：我们建立了一个模块化 PPSDHM，它能够通过将 PPSDHM 的光学元件集成到光学面包板上来获取 3D 图像。PPSDHM 通过实验证明了动态标本的 3D 运动图像的记录能力。我们建立了一个模块化 PPSDHM，它能够通过将 PPSDHM 的光学元件集成到光学面包板上来获取 3D 图像。PPSDHM 通过实验证明了动态标本的 3D 运动图像的记录能力。我们建立了一个模块化 PPSDHM，它能够通过将 PPSDHM 的光学元件集成到光学面包板上来获取 3D 图像。PPSDHM 通过实验证明了动态标本的 3D 运动图像的记录能力。