(1) Background: Access to clean water is a very important factor for human life. However, pathogenic microorganisms in drinking water often cause diseases, and convenient/inexpensive testing methods are urgently needed. (2) Methods: The reagent contains 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and phenazine methosulfate (PMS) and can react with succinate dehydrogenase within bacterial cell membranes to produce visible purple crystals. The colorimetric change of the reagent after reaction can be measured by a sensor (AS7262). (3) Results: Compared with traditional methods, our device is simple to operate and can provide rapid (i.e., 5 min) semi-quantitative results regarding the concentration of bacteria within a test sample. (4) Conclusions: This easy-to-use device, which employs MTT-PMS reagents, can be regarded as a potential and portable tool for rapid water quality determination.

中文翻译：

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用于快速检测水中活菌的便携式设备

（1）背景：获得清洁水是人类生活的重要因素。然而，饮用水中的病原微生物通常会引起疾病，因此迫切需要方便/廉价的测试方法。（2）方法：该试剂包含3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）和吩嗪硫酸甲酯（PMS），可与细菌细胞膜中的琥珀酸脱氢酶反应产生可见的紫色晶体。反应后试剂的比色变化可以通过传感器（AS7262）测量。（3）结果：与传统方法相比，我们的设备操作简单，并且可以提供有关测试样品中细菌浓度的快速（即5分钟）半定量结果。（4）结论：这种易于使用的设备采用了MTT-PMS试剂，