ABSTRACT

DNA methylation plays key roles in regulating genes which control somatic embryogenesis (SE) in plant. 5-Azacytidine (5-AzaC) was considered as a common inhibitor of DNA methylation. Longan (Dimocarpus longan Lour.) SE has been established and used as model system for studying mechanism of SE in woody plants. However, effects of 5-AzaC on longan EC and the underlying mechanism at molecular level are rarely understood. In this study, RNA-seq was performed for transcriptome analysis of longan EC treated with or without 5-AzaC were analysed. A total of 29,232 expressed genes were obtained by aligned to longan reference genome and 1,617 differentially expressed genes (DEGs) were got in the pairwise comparisons of CK_vs_T. Integrating the KEGG enrichment pathway analysis showed that ‘butanoate metabolism’, ‘C5-branched dibasic acid metabolism’, ‘sulphur metabolism’, ‘selenocompound metabolism’, ‘pantothenate and CoA biosynthesis’, ‘phagosome’, and ‘plant hormone signal transduction’ were significantly enriched pathways, while the analysis of DEGs revealed that 5-AzaC-treatment could alter genes expression which related to the SE to adjust the complicated metabolic pathways. Therefore, these results indicated that 5-AzaC-treatment positively impact the development of early SE from longan and a decrease in the global DNA methylation level during early SE in longan.

摘要

DNA甲基化在调节控制植物体胚发生（SE）的基因中起着关键作用。5-氮杂胞苷（5-AzaC）被认为是DNA甲基化的常见抑制剂。龙眼（Dimocarpus longan Lour。）SE已经建立，并用作研究木本植物中SE机理的模型系统。然而，很少了解5-AzaC对龙眼EC的影响以及在分子水平上的潜在机制。在这项研究中，进行了RNA-seq的转录组分析，以分析经或未经5-AzaC处理的龙眼EC。通过与龙眼参考基因组比对获得总共29,232个表达基因，并且在CK_与vs的成对比较中获得1,617个差异表达基因（DEG）。_T。整合KEGG富集途径分析显示，``丁酸代谢''，``C5支二元酸代谢''，``硫代谢''，``硒化合物代谢''，``泛酸和CoA生物合成''，``吞噬体''和``植物激素信号转导''它们是显着富集的途径，而DEG的分析显示5-AzaC处理可以改变与SE相关的基因表达，从而调节复杂的代谢途径。因此，这些结果表明5-AzaC处理对龙眼早期SE的发展有积极影响，并且对龙眼SE早期的整体DNA甲基化水平的降低有积极的影响。