Circadian clock operates autonomously in each cell and drives the approximately 24-h rhythm in individual tissues and organs. It is known that the evening complex (EC) components and GI are required for ambient temperature perception and thermomorphogenesis in higher plants. Our previous study found that PRR9 and 7 are required for the lengthened period of the circadian rhythm in roots, and they are responsible for the temperature overcompensation in shoots. However, the molecular mechanism of the circadian clock, especially in different tissues, in response to temperature oscillations remains largely unknown. Here, we studied the transcript levels of EC genes and GI of the prr7 prr9 mutant shoots and roots in response to 22°C or 28°C, respectively. The results showed that PRR9, 7 in roots inhibited the transcripts accumulation of ELF3, ELF4, and LUX at 28°C. In addition, loss-of-function of both PRR9 and 7 caused an increase in GI expression at 22°C, but warm temperature of 28°C limited the negative effect of PRR9, 7 on GI in roots. Our findings proposed a temperature-dependent molecular basis for root-specific circadian clock and indicated the critical role for PRR9, 7 in negatively regulating ELF3, ELF4, LUX, and GI in the circadian gating of thermoresponse.

中文翻译：

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PRR9和PRR7负调控根中EC组分的表达

生物钟在每个细胞中自主运行，并在个体组织和器官中驱动大约 24 小时的节律。众所周知，高等植物的环境温度感知和热形态发生需要晚间复合物 (EC) 成分和 GI。我们之前的研究发现，PRR9 和 7 是根中昼夜节律延长周期所必需的，它们是造成枝条温度过度补偿的原因。然而，生物钟的分子机制，尤其是在不同组织中，对温度振荡的反应在很大程度上仍然未知。在这里，我们研究了 prr7 prr9 突变体枝条和根分别响应 22°C 或 28°C 的 EC 基因和 GI 的转录水平。结果表明，根中的PRR9、7抑制了ELF3、ELF4、和 LUX 在 28°C。此外，PRR9 和 7 的功能丧失导致 22°C 时 GI 表达增加，但 28°C 的温暖温度限制了 PRR9, 7 对根中 GI 的负面影响。我们的研究结果提出了根特异性生物钟的温度依赖性分子基础，并表明 PRR9, 7 在负调节 ELF3、ELF4、LUX 和 GI 中的热反应昼夜节律门控中的关键作用。