Abstract To evaluate the protective effects of dietary resveratrol (RES) supplementation against oxidative stress in piglets, a total of 30 weaned piglets (aged 28 + 2 d) were randomly divided into five treatments (n = 6): C group (basal diet), NC group (basal diet + diquat challenge), and basal diet containing either 10, 30, or 90 mg/kg of RES administered to piglets injected with diquat. The trail lasted 21 d. On day 15 after the initiation of treatment, piglets were intraperitoneally injected with 10 mg/kg body weight (BW) diquat or the same amount of saline. The feed intake and faecal consistency were recorded daily. Blood samples were collected for antioxidative, immune parameters and cytokines analysis. RES addition showed a negative tendency (p = .052) on faecal score during the pre-challenge period. Diquat challenge reduced the growth performance and induced oxidative stress and inflammatory responses of piglets (p < .05). However, the growth performance was improved, and faecal score was decreased in 90 mg/kg RES groups (p < .05). Compared with the NC group, addition of 90 mg/kg RES increased activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC), and reduced malonaldehyde (MDA), cortisol and adrenocorticotropic hormone (ATCH) concentration (p < .05). Moreover, supplementation with 90 mg/kg RES increased serum concentration of interleukin-4 (IL-4) (p < .05) but decreased serum tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) (p < .05). In conclusion, the study indicated that RES showed overwhelming advantage in reducing diarrhoea and attenuating the oxidative stress of piglets challenged with diquat. Highlights Dietary resveratrol supplementation improved growth performance and reduce diarrhoea of piglets challenged with diquat. The protective effects of dietary resveratrol supplementation against oxidative stress and inflammation in diquat-challenged piglets were confirmed. The experimental results provide theoretical and practical value for further research on the antioxidant mechanisms of resveratrol in weaned piglets.

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日粮添加白藜芦醇对敌草快攻击仔猪氧化应激的保护作用

摘要 为评价日粮添加白藜芦醇（RES）对仔猪氧化应激的保护作用，将30头断奶仔猪（28+2天）随机分为5个处理组（n=6）：C组（基础日粮） 、NC 组（基础日粮 + 敌草快攻击）和含有 10、30 或 90 mg/kg RES 的基础日粮给予注射敌草快的仔猪。跟踪持续了21 d。在开始治疗后第 15 天，仔猪腹腔注射 10 mg/kg 体重 (BW) 敌草快或等量生理盐水。每天记录采食量和粪便稠度。收集血样用于抗氧化、免疫参数和细胞因子分析。在攻击前期间，RES 添加对粪便评分显示出负面趋势 (p = .052)。敌草快攻击降低了仔猪的生长性能并诱导了氧化应激和炎症反应（p < .05）。然而，90 mg/kg RES 组的生长性能得到改善，粪便评分降低（p < .05）。与 NC 组相比，添加 90 mg/kg RES 增加了超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH-Px) 和总抗氧化能力 (T-AOC) 的活性，并降低了丙二醛 (MDA)、皮质醇和促肾上腺皮质激素激素 (ATCH) 浓度 (p < .05)。此外，补充 90 mg/kg RES 增加了白细胞介素 4 (IL-4) 的血清浓度 (p < .05)，但降低了血清肿瘤坏死因子-α (TNF-α)、白介素 6 (IL-6)、和白细胞介素-1β (IL-1β) (p < .05)。综上所述，该研究表明，RES 在减少敌草快攻击的仔猪腹泻和减轻氧化应激方面显示出压倒性优势。亮点 日粮中添加白藜芦醇可改善敌草快感染仔猪的生长性能并减少其腹泻。证实了在敌草快攻击的仔猪中添加白藜芦醇对氧化应激和炎症的保护作用。实验结果为进一步研究白藜芦醇在断奶仔猪中的抗氧化机制提供了理论和实用价值。证实了在敌草快攻击的仔猪中添加白藜芦醇对氧化应激和炎症的保护作用。实验结果为进一步研究白藜芦醇在断奶仔猪中的抗氧化机制提供了理论和实用价值。证实了在敌草快攻击的仔猪中添加白藜芦醇对氧化应激和炎症的保护作用。实验结果为进一步研究白藜芦醇在断奶仔猪中的抗氧化机制提供了理论和实用价值。