The rise of antimicrobial resistant bacteria has fast-tracked the exploration for novel antimicrobial compounds. Reports on antimicrobial producing soil anaerobes such as Clostridium spp. are very limited. In the present study, the antimicrobial activity of soil Clostridium enriched conditioned/spent media (CMs) against Bacillus mycoides, Bacillus cereus and Pseudomonas aeruginosa was assessed by turbidimetric growth inhibition assay. Our results highlighted the antimicrobial potential of soil Clostridium enriched conditioned media against pathogenic and spoilage bacteria. Farm 4 soil conditioned medium (F4SCM) demonstrated a greater growth inhibition activity against all three tested microorganisms in comparison to other soil conditioned media. Non-targeted metabolite profiling of all soil conditioned media revealed distinctive polar and intermediate-polar metabolites in F4SCM, consistent with its strong antimicrobial property. Moreover, 539 significantly abundant metabolites including some unique features were detected in F4SCM suggesting its substantial and specialized chemical diversity. This study putatively identified seven significantly high metabolites in F4SCM; 3-hydroxyphenylacetic acid, γ-aminobutyric acid, creatine, tryptamine, and 2-hydroxyisocaproic acid. Tryptamine and 2-hydroxyisocaproic acid were previously reported to have antimicrobial properties. The present study shows that soil Clostridium spp. are a promising group of bacteria producing metabolites with antimicrobial activity and provides future prospects for clostridial antimicrobial discovery within their metabolic diversity.

抗菌素耐药菌的兴起已迅速探索了新型抗菌素化合物。有关产生微生物的土壤厌氧菌的报告，例如梭菌spp。非常有限。在本研究中，土壤的抗菌活性梭菌 丰富的条件/耗材（CM） 芽孢杆菌， 蜡状芽孢杆菌 和 铜绿假单胞菌通过比浊生长抑制测定法评估。我们的结果突出了土壤的抗菌潜力梭菌丰富的条件培养基可抵抗致病菌和腐败菌。与其他土壤条件培养基相比，Farm 4土壤条件培养基（F4SCM）对所有三种测试微生物表现出更大的生长抑制活性。所有土壤条件培养基的非靶向代谢物谱图显示，F4SCM中具有独特的极性和中间极性代谢物，与其强大的抗菌特性相符。此外，在F4SCM中检测到539种显着丰富的代谢物，包括一些独特的特征，表明其大量且专门的化学多样性。这项研究推定了F4SCM中的7种显着高的代谢产物。3-羟基苯基乙酸，γ-氨基丁酸，肌酸，色胺和2-羟基异己酸。以前有报道称色胺和2-羟基异己酸具有抗菌性能。本研究表明土壤梭菌spp。是一组有前途的细菌，其产生具有抗微生物活性的代谢产物，并为在其代谢多样性内发现梭菌抗微生物剂提供了未来前景。