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Microsatellites for the Neotropical ant, Camponotus leydigi (Hymenoptera: Formicidae)
Entomological Science ( IF 0.7 ) Pub Date : 2020-12-03 , DOI: 10.1111/ens.12454 Salatiel Gonçalves‐Neto 1 , Marianne Azevedo‐Silva 2 , Alessandra S. M. Lemos 1 , Anete P. Souza 3 , Paulo S. Oliveira 4
Entomological Science ( IF 0.7 ) Pub Date : 2020-12-03 , DOI: 10.1111/ens.12454 Salatiel Gonçalves‐Neto 1 , Marianne Azevedo‐Silva 2 , Alessandra S. M. Lemos 1 , Anete P. Souza 3 , Paulo S. Oliveira 4
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Ants (Hymenoptera: Formicidae) are dominant social insects that play important ecological roles in terrestrial ecosystems. Camponotus leydigi (Forel) is widely distributed in the Neotropical region and is frequently found in the Brazilian cerrado savanna interacting with plants and other insects. Field observations indicate that C. leydigi has a polydomous nesting habit, but little is known about the genetic relationship among workers. In this study, we identify the first nine microsatellite loci for C. leydigi that will allow further investigation on its genetic diversity. We used a microsatellite‐enriched library method. According to this method, repetitive sequences are captured with (CT)8 and (GT)8 biotin‐linked probes, with subsequent recovery by streptavidin magnetic‐coated beads. We observed that eight loci were polymorphic. The mean (± standard error) observed and expected heterozygosities were 0.55 ± 0.23 and 0.73 ± 0.28, respectively. The rarified allelic richness ranged from 1 to 5.32. The polymorphism contents were similar to diversity estimates found in markers previously developed for other Camponotus ants. These markers will be useful for future studies on population genetics and ecology of Camponotus ants in cerrado, including nesting ecology, colony structure, dispersal and conservation.
中文翻译：
新型蚂蚁，Camponotus leydigi的微卫星（膜翅目：mic科）
蚂蚁（膜翅目：蚁科）是占主导地位的社会昆虫，在陆地生态系统中起着重要的生态作用。Camponotus leydigi（Forel）广泛分布于新热带地区，并经常在与植物和其他昆虫相互作用的巴西cerrado稀树草原中发现。现场观察表明，C。leydigi有多巢的习性，但对工人之间的遗传关系知之甚少。在这项研究中，我们确定了C. leydigi的前9个微卫星基因座，这将允许对其遗传多样性进行进一步的研究。我们使用了富含微卫星的文库方法。根据此方法，用（CT）8和（GT）8捕获重复序列生物素连接的探针，随后可通过抗生蛋白链菌素磁性包被的磁珠回收。我们观察到八个基因座是多态的。观察到的平均杂合度（±标准误）和预期杂合度分别为0.55±0.23和0.73±0.28。稀有的等位基因丰富度范围为1到5.32。多态性含量类似于先前为其他Camponotus蚂蚁开发的标记中发现的多样性估计。这些标记物可用于今后对塞拉多Camponotus蚂蚁的种群遗传学和生态学研究，包括巢式生态学，菌落结构，传播和保护等。
更新日期：2021-02-02
中文翻译：
新型蚂蚁，Camponotus leydigi的微卫星（膜翅目：mic科）
蚂蚁（膜翅目：蚁科）是占主导地位的社会昆虫，在陆地生态系统中起着重要的生态作用。Camponotus leydigi（Forel）广泛分布于新热带地区，并经常在与植物和其他昆虫相互作用的巴西cerrado稀树草原中发现。现场观察表明，C。leydigi有多巢的习性，但对工人之间的遗传关系知之甚少。在这项研究中，我们确定了C. leydigi的前9个微卫星基因座，这将允许对其遗传多样性进行进一步的研究。我们使用了富含微卫星的文库方法。根据此方法，用（CT）8和（GT）8捕获重复序列生物素连接的探针，随后可通过抗生蛋白链菌素磁性包被的磁珠回收。我们观察到八个基因座是多态的。观察到的平均杂合度（±标准误）和预期杂合度分别为0.55±0.23和0.73±0.28。稀有的等位基因丰富度范围为1到5.32。多态性含量类似于先前为其他Camponotus蚂蚁开发的标记中发现的多样性估计。这些标记物可用于今后对塞拉多Camponotus蚂蚁的种群遗传学和生态学研究，包括巢式生态学，菌落结构，传播和保护等。
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