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ATP‐dependent RNA helicase domain of the ZC3H41 protein from Trypanosoma brucei: expression, purification and crystallization
Acta Crystallographica Section F ( IF 1.1 ) Pub Date : 2020-12-04 , DOI: 10.1107/s2053230x20015204
Lee S Izhaki-Tavor 1 , Moshe Dessau 1
Affiliation  

A fragment of the Trypanosoma brucei ZC3H41 protein encompassing the ATP‐dependent RNA helicase domain was successfully subcloned for expression in a bacterial system (Escherichia coli). Following expression, the protein was purified and crystallized using the vapor‐diffusion method. The protein crystals were optimized at a 1:1 protein:reservoir solution ratio using PPGBA 2000. The optimized crystals diffracted to a dmin of 3.15 Å. The collected data revealed preliminary structural information regarding this newly discovered protein.

中文翻译:

来自布氏锥虫的 ZC3H41 蛋白的 ATP 依赖性 RNA 解旋酶结构域:表达、纯化和结晶

包含 ATP 依赖性 RNA 解旋酶结构域的布氏锥虫ZC3H41 蛋白片段被成功亚克隆,用于在细菌系统(大肠杆菌)中表达。表达后,使用蒸气扩散法纯化和结晶蛋白质。使用 PPGBA 2000 以 1:1 的蛋白质:储备溶液比例优化蛋白质晶体。优化后的晶体衍射至3.15 Å的d min。收集到的数据揭示了有关这种新发现蛋白质的初步结构信息。
更新日期:2020-12-04
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