Despite a large number of wound dressing materials in the market, an ideal dressing able to re‐establish both the features and functions of the native skin remains a challenge. Concerning this, the present study aimed to produce a new double‐layer nanofibrous dressing material. The upper layer was fabricated by using Poly(l‐lactic acid; PLLA) to act as a hydrophobic protective barrier, while the highly porous lower layer composed of a blend of Poly(ethylene) oxide (PEO) and Chitosan (CS) containing Hypericum perforatum L. (HP) was produced to be used in the wound site, reduce the risk of infection, and enhance the healing process. The produced double‐layer material exhibited the required chemical, physical, and mechanical properties. Besides, the results showed an inhibitory effect on Staphylococcus aureus and Pseudomonas aeruginosa growth without providing any cytotoxicity on normal human dermal fibroblast (NHDF) cells. Therefore, these findings emphasize the potential use of this material as an antimicrobial wound dressing to treat skin lesions.

中文翻译：

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含有贯叶连翘的双层PLLA / PEO_壳聚糖纳米纤维垫作为伤口治疗的有效方法

尽管市场上有大量伤口敷料，但要能够重建天然皮肤的特征和功能，理想的敷料仍然是一个挑战。关于这一点，本研究旨在生产一种新型的双层纳米纤维敷料。上层是通过使用聚（l-乳酸； PLLA）充当疏水性保护屏障而制成的，而高度多孔的下层则是由含有金丝桃属植物的聚环氧乙烷（PEO）和壳聚糖（CS）的混合物组成的贯叶连翘L.（HP）被生产用于伤口部位，降低感染风险，并增强愈合过程。所生产的双层材料具有所需的化学，物理和机械性能。此外，结果显示对金黄色葡萄球菌和铜绿假单胞菌的生长具有抑制作用，而对正常人真皮成纤维细胞（NHDF）没有任何细胞毒性。因此，这些发现强调了这种材料作为治疗皮肤损伤的抗菌伤口敷料的潜在用途。