Epitranscriptomic m6A regulation following spinal cord injury,Journal of Neuroscience Research

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Epitranscriptomic m6A regulation following spinal cord injury
Journal of Neuroscience Research ( IF 2.9 ) Pub Date : 2020-12-03 , DOI: 10.1002/jnr.24763
Lingyan Xing ₁ , Yunyun Cai ₂ , Tuo Yang ₃ , Weiwei Yu ₁ , Mengdie Gao ₁ , Rui Chai ₁ , Sujun Ding ₄ , Jinhuan Wei ₅ , Jingying Pan ₆ , Gang Chen _{6,

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Affiliation

RNA methylation is involved in multiple physiological and pathological processes. However, the role of RNA methylation in spinal cord regeneration has not been reported. In this study, we find an altered m6A (N6‐methyladenosine) RNA methylation profiling following zebrafish spinal cord injury (SCI), in line with an altered transcription level of the m6A methylase Mettl3. Interestingly, many of the differential m6A‐tagged genes associated with neural regeneration are hypomethylated, but their transcription levels are upregulated in SCI. Moreover, we find that METTL3 may be important for spinal cord regeneration. We also show a conserved feature of METTL3 changes in mouse SCI model, in which the expression of METTL3 is increased in both astrocytes and neural stem cells. Together, our results indicate that m6A RNA methylation is dynamic and conserved following SCI and may contribute to spinal cord regeneration.

中文翻译：

脊髓损伤后的表观转录组 m6A 调节

RNA甲基化参与多种生理和病理过程。然而，RNA甲基化在脊髓再生中的作用尚未见报道。在这项研究中，我们发现斑马鱼脊髓损伤 (SCI) 后 m6A (N6-甲基腺苷) RNA 甲基化谱的改变，与 m6A 甲基化酶 Mettl3 的转录水平改变一致。有趣的是，许多与神经再生相关的差异 m6A 标记基因是低甲基化的，但它们的转录水平在 SCI 中上调。此外，我们发现 METTL3 可能对脊髓再生很重要。我们还显示了小鼠 SCI 模型中 METTL3 变化的保守特征，其中 METTL3 的表达在星形胶质细胞和神经干细胞中均增加。一起，

更新日期：2021-01-29

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