The applications of green synthesised gold nanoparticles for the biomedical field are the most prominent and developing area in this modern era. In this present investigation, marine bacteria, Vibrio alginolyticus was used for the biosynthesis of gold nanoparticles. The biosynthesized gold nanoparticles (AuNps) were characterized by various spectroscopic analysis. The morphological analysis of AuNps was done through scanning electron microscope (SEM) and transmission electron microscope (TEM) respectively. The obtained size was estimated around 100–150 nm. The synthesized AuNps confirmed by its surface plasmon resonance peak (SPR) range using UV–Vis spectrophotometer. The bio-metabolites from marine bacteria which was responsible for the nanoparticles synthesis were analyzed using fourier transform infrared spectrophotometer (FT-IR). The biomedical application of synthesized eco-friendly AuNps analysed by different method such as, the antioxidant property and free radical scavenging activity through DPPH (1, 1- diphenyl 2-picrylhyorazyl) and metal chelating assay. The anticancer activity of colon carcinoma was confirmed through MTT assay while the apoptotic mediated cell death observed through the fluorescence analysis. The biosynthesized AuNPs showed a dose dependent inhibitory effect on the growth of colon cancer cells. The IC50 was 15 µg/ mL, and the maximum inhibition of the cell death was (>75%) obtained when treating 25 µg/mL. The apoptotic mediated cell death observed through nuclear condensation of the treated cells. The green synthesis of AuNPs emphases - to be cost effective and environmental friendly with anti-inflammatory and anti-cancer effects against colon cancer.

绿色合成的金纳米颗粒在生物医学领域的应用是这个现代时代最突出和发展的领域。在本次调查中，海洋细菌溶藻弧菌用于金纳米颗粒的生物合成。通过各种光谱分析对生物合成的金纳米颗粒（AuNps）进行了表征。分别通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）对AuNps进行了形态分析。估计获得的尺寸约为100–150 nm。使用紫外可见分光光度计通过表面等离子体共振峰（SPR）范围确认了合成的AuNps。使用傅里叶变换红外分光光度计（FT-IR）分析了负责海洋生物合成的海洋细菌生物代谢产物。合成的环保型AuNps的生物医学应用通过不同的方法进行了分析，例如通过DPPH的抗氧化性能和自由基清除活性（1，1-二苯基2-吡咯并吡嗪）和金属螯合测定。通过MTT测定证实了结肠癌的抗癌活性，而通过荧光分析观察到了凋亡介导的细胞死亡。生物合成的AuNPs对结肠癌细胞的生长表现出剂量依赖性的抑制作用。IC50为15 µg / mL，处理25 µg / mL时，对细胞死亡的最大抑制作用（> 75％）。通过处理的细胞的核浓缩观察到凋亡介导的细胞死亡。AuNPs的绿色合成非常重要-具有成本效益和环境友好性，并且具有抗结肠癌的抗炎和抗癌作用。生物合成的AuNPs对结肠癌细胞的生长表现出剂量依赖性的抑制作用。IC50为15 µg / mL，处理25 µg / mL时，对细胞死亡的最大抑制作用（> 75％）。通过处理的细胞的核浓缩观察到凋亡介导的细胞死亡。AuNPs的绿色合成非常重要-具有成本效益和环境友好性，并且具有抗结肠癌的抗炎和抗癌作用。生物合成的AuNPs对结肠癌细胞的生长表现出剂量依赖性的抑制作用。IC50为15 µg / mL，处理25 µg / mL时，对细胞死亡的最大抑制作用（> 75％）。通过处理的细胞的核浓缩观察到凋亡介导的细胞死亡。AuNPs的绿色合成非常重要-具有成本效益和环境友好性，并且具有抗结肠癌的抗炎和抗癌作用。