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Using a radiopaque marker with radiography for evaluating colonic transit by geometric center in conscious rats: A novel method
Autonomic Neuroscience ( IF 3.2 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.autneu.2020.102760
Misaki Okada 1 , Sazu Taniguchi 2 , Chiaki Takeshima 3 , Hiroshi Taniguchi 4 , Hiroshi Kitakoji 5 , Kazunori Itoh 1 , Toku Takahashi 6 , Kenji Imai 7
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This study developed a new method using radiopaque markers under X-ray to measure rat colonic transit by geometric center repeatedly and/or over a time series in the same individually. Additionally, the utility of this method was shown by elucidating the innervation of the autonomic nerve on colonic transit in detail with a pharmacological technique in conscious rats. An in-dwelling silastic cannula was inserted into the cecum and the proximal part was moved through the abdominal wall, where it was fixed to the posterior neck skin. Twenty markers were administered from the cannula to the proximal colon with saline on the fifth day after surgery. The markers were observed with soft X-ray before required repeated short anesthesia. Experimentation 1: Rats were measured colonic transit twice over 2 days with no administration. Experimentation 2: Rats were administered saline on the first day and pharmacology on the second day intraperitoneally before measurement. Experimentation 1: The markers administrated from the cannula and transited from proximal colon to distal colon over a time series. It showed no significant difference in complication rates between 2 days. Experimentation 2: The colonic transit was increasingly accelerated by neostigmine and phentolamine but not propranolol. Significant changes in 1.0 mg/kg atropine were noted although no differences were found between control and 0.05 mg/kg atropine and between each other's. We have presented the method using radiopaque markers under X-ray with short anesthesia for evaluating the colonic transit. The methods could show rat colonic transit changes in detail with a pharmacological technique.

中文翻译:

使用射线不透标记和放射照相术评估清醒大鼠几何中心的结肠转运:一种新方法

这项研究开发了一种新方法,在 X 射线下使用不透射线标记,通过几何中心重复和/或在同一时间序列中单独测量大鼠结肠传输。此外,通过在清醒大鼠中使用药理学技术详细阐明自主神经对结肠传输的神经支配,显示了该方法的实用性。将留置的硅橡胶套管插入盲肠,将近端部分移过腹壁,固定在颈后皮肤上。在手术后的第五天,用盐水从套管向近端结肠施用 20 个标记物。在需要反复短暂麻醉之前,用软 X 射线观察标记。实验1:在没有给药的情况下,在2天内对大鼠进行两次结肠传输测量。实验二:在测量前的第一天和第二天腹膜内给大鼠施用生理盐水。实验 1:标记物从套管给药并在一个时间序列内从近端结肠转移到远端结肠。它显示2天之间的并发症发生率没有显着差异。实验 2:新斯的明和酚妥拉明但不是普萘洛尔加速了结肠传输。尽管对照和 0.05 mg/kg 阿托品之间以及彼此之间没有发现差异,但注意到 1.0 mg/kg 阿托品的显着变化。我们提出了在 X 射线下使用不透射线标记物和短时麻醉来评估结肠传输的方法。该方法可以通过药理学技术详细显示大鼠结肠传输的变化。
更新日期:2021-01-01
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