This work aimed to evaluate the ability of Sporothrix species to attach and form biofilm on the surface of cat claws as an ex vivo model. A total of 14 strains (5 Sporothrix brasiliensis, 3 Sporothrix schenckii s. str., 3 Sporothrix globosa and 3 Sporothrix mexicana) were used. The biofilms were incubated for periods of 01, 03, 07, 10 and fifteenth 15 days. Their metabolic activities were evaluated by the XTT reduction assay and the morphology and structure were investigated by scanning electron microscopy (SEM). The analysis of the SEM images revealed that all the species can form biofilms on cat claws. The metabolic activity in the ex vivo biofilms was similar to that found in in vitro biofilms when incubated for the same period. This is the first report of an ex vivo biofilm model involving cat claws. The ability to form biofilms on cat claws can increase the viable period of the fungus and consequently the number of possibly infected animals and people.

这项工作旨在评估作为体外模型，孢子菌属物种在猫爪表面附着并形成生物膜的能力。总共使用了14个菌株（5个巴西孢子菌，3个sporothrix schenckii s.str。，3个球形孢子和3个墨西哥孢子）。将生物膜温育01、03、07、10和第15 15天。通过XTT还原法评估其代谢活性，并通过扫描电子显微镜（SEM）研究其形态和结构。扫描电镜图像分析表明，所有物种都可以在猫爪上形成生物膜。离体代谢活动当孵育相同的时间时，生物膜类似于在体外生物膜中发现的生物膜。这是涉及猫爪的离体生物膜模型的首次报道。在猫爪上形成生物膜的能力可以延长真菌的生存期，从而增加可能感染的动物和人的数量。