Polycyclic aromatic hydrocarbons (PAHs) such as pyrene are universal contaminants existing in the environment which have known cancer-causing and mutagenic characteristics. A psychrophilic bacterial strain Shewanella sp. ISTPL2 was isolated from the sediment sample collected from the Pangong lake, Jammu & Kashmir, India. In our previous study, the pyrene degradation potential of the ISTPL2 strain was studied in both mineral salt media as well as in soil artificially spiked with different concentrations of pyrene. Whole-genome sequencing of ISTPL2 strain in the current study highlighted the key genes of pyrene metabolism, including alcohol dehydrogenase and ring hydroxylating dioxygenase alpha-subunit. Pyrene cytotoxicity was evaluated on HepG2, a human hepato-carcinoma cell line. The cytotoxicity of the organic extract decreased with the increasing duration of bacterial treatment. To develop a more sustainable biodegradation approach, the potential impacts were evaluated for human health and ecosystem using life-cycle assessment (LCA) following the ReCiPe methodology for the considered PAH. The results implemented that global warming potential (GWP) had the highest impact, whereas both ecotoxicity and human toxicity had least from this study.

cyclic等多环芳烃（PAH）是存在于环境中的普遍污染物，具有已知的致癌和诱变特性。嗜热细菌希瓦氏菌sp。ISTPL2是从印度查Jam和克什米尔邦公湖收集的沉积物样品中分离出来的。在我们先前的研究中，在矿物盐介质以及人工掺入不同浓度pyr的土壤中都研究了ISTPL2菌株的the降解潜力。在目前的研究中，ISTPL2菌株的全基因组测序突出了of代谢的关键基因，包括醇脱氢酶和环羟化双加氧酶α-亚基。在人肝癌细胞系HepG2上评估了的细胞毒性。有机提取物的细胞毒性随着细菌处理时间的延长而降低。为了开发更可持续的生物降解方法，根据ReCiPe方法对所考虑的PAH进行生命周期评估（LCA），评估对人类健康和生态系统的潜在影响。该结果表明，全球变暖潜势（GWP）影响最大，而这项研究对生态毒性和人类毒性影响最小。