Abstract — Deep biosphere is an prolific source of novel prokaryotes. A phylogenetically distant lineage of Firmicutes was reported in 2019 as a new genus Thermoanaerosсeptrum . Ecology of this group remains uncertain, since the only known species, T. fracticalcis , gains energy from the use of fumarate, and the source of the latter in groundwater is not apparent. The organism is incapable of using any of the oxidized sulfur species (sulfate, sulfite, thiosulfate, or elemental sulfur) as an electron acceptor for respiration, although the proteins required for dissimilatory sulfate reduction are present in the genome. Research on T. fracticalcis ecology is complicated by the fact that the organism constitutes a minor component of the microbial community. The present work was aimed at the use of microbial mats formed at the head of a deep thermal borehole to obtain spore-forming sulfidogens. The isolated pure culture, Thermoanaerosсeptrum sp. BuN1, reduced sulfate and exhibited saccharolytic activity. It could grow within a broad pH range (3.5 to 9). Strain BuN1 was a moderate thermophile growing within the range from 37 to 60°C, with an optimum at 50°C.

中文翻译：

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微生物垫在分离深部生物圈产孢原核生物中的应用

摘要 — 深层生物圈是新型原核生物的丰富来源。2019 年报道了一个系统发育上较远的厚壁菌门谱系，称为 Thermoanaerosceptrum 新属。这个群体的生态仍然不确定，因为唯一已知的物种 T. fracticalcis 通过使用富马酸盐获得能量，而后者在地下水中的来源并不明显。尽管基因组中存在异化硫酸盐还原所需的蛋白质，但生物体不能使用任何氧化硫物质（硫酸盐、亚硫酸盐、硫代硫酸盐或元素硫）作为呼吸的电子受体。由于有机体构成微生物群落的次要组成部分，因此对 T. fracticalcis 生态学的研究变得复杂。目前的工作旨在利用在深热钻孔顶部形成的微生物垫来获得形成孢子的硫化物。分离的纯培养物 Thermoanaerosceptrum sp。BuN1，还原硫酸盐并表现出糖分解活性。它可以在很宽的 pH 值范围内（3.5 到 9）生长。菌株 BuN1 是在 37 到 60°C 范围内生长的中等嗜热菌，最适温度为 50°C。