Tuz Lake is a shallow, perennial, inland hypersaline lake located at the center of the Turkey. Microbial populations developing in the sedimentary layers of the lake were investigated by using 16S rRNA gene-targeted Illumina MiSeq sequencing and cloning. A total of 11 samples were taken from three sampling points. Here we report the relative abundance of microbial taxa distributed across the sediment samples. Abundant, rare and region-specific prokaryotic taxa were also examined and compared. The majority of high-throughput sequences were associated with the classes Halobacteria, Gammaproteobacteria, Bipolaricaulia, Desulfovibrionia, candidate division MSBL-1, Bacteroidia, and Desulfobacteria. Gemmatimonadota_c, Rhodothermia, Alphaproteobacteria, Methanonatronarchaeia, Thermoplasmata, Halanaerobiia, Desulfobulbia, and Bacilli were other notable taxa with low abundance. A genus belonging to Bipolaricaulia was the most abundant taxon, accounting for 11.5% of all MiSeq reads. Reads affiliated with the genera Halomonas, Desulfovermiculus, MSBL-1_g1, and Halodesulfurarchaeum were also detected at high abundance. Bipolaricaulota, Bacteroidales, Desulfosalsimonas, Desulfobulbales, Gemmatimonadota, Lentisphaeria, Halobacteriales, MSBL-1, Thermoplasmata, and DHVEG-1 related phylotypes were also frequently represented in the clone libraries.

中文翻译：

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克隆和高通量测序证明图兹湖高盐度沉积物的原核群落组成

图兹湖是位于土耳其中部的一个多年生浅水内陆高盐度湖。通过使用 16S rRNA 基因靶向 Illumina MiSeq 测序和克隆研究在湖泊沉积层中发育的微生物种群。从三个采样点共抽取了11个样本。在这里，我们报告了分布在沉积物样本中的微生物类群的相对丰度。还检查和比较了丰富的、稀有的和区域特异性的原核生物分类群。大多数高通量序列与 Halobacteria、Gammaproteobacteria、Bipolaricaulia、Desulfovibrionia、候选分类 MSBL-1、Bacteroidia 和 Desulfobacteria 相关。Gemmatimonadota_c, Rhodothermia, Alphaproteobacteria, Methanonatronarchaeia, Thermoplasmata, Halanaerobiia, Desulfobulbia, 和 Bacilli 是其他显着的低丰度分类群。属于 Bipolaricaulia 的一个属是最丰富的分类群，占所有 MiSeq 读数的 11.5%。与 Halomonas、Desulfovermiculus、MSBL-1_g1 和 Halodesulfurarchaeum 属相关的读数也被检测到高丰度。Bipolaricaulota、Bacteroidales、Desulfosalsimonas、Desulfobulbales、Gemmatimonadota、Lentisphaeria、Halobacteriales、MSBL-1、Thermoplasmata 和 DHVEG-1 相关系统发育型也经常出现在克隆库中。