Abstract—

Methane oxidation rates and diversity of methane-oxidizing microorganisms in the upper sediment layers of the Crimean Peninsula coastal regions (Black Sea) were investigated. Analysis of the 16S rRNA gene sequences revealed microorganisms of the class Gammaproteobacteria, order Methylococcales. At the sites of continuous gas seepage, the percentage of methanotrophs in the bacterial community was high (up to 2.1% of the total read number), which made it possible to detect them by sequencing of the native samples. At the sites of intermittent gas seepage formed during summer and autumn, methanotrophs were minor components of the communities and could be detected only after incubation of the sediment samples in the presence of methane. Low similarity between their 16S rRNA sequences and those of the known methanotrophs indicates that some organisms probably belong to new taxa of the species and genus levels.

中文翻译：

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克里米亚半岛沿海沉积物中好氧甲烷氧化细菌的鉴定

摘要—

研究了克里米亚半岛沿海地区（黑海）上层沉积层的甲烷氧化速率和甲烷氧化微生物的多样性。16S rRNA基因序列的分析揭示了类的微生物γ-变形菌，为了Methylococcales。在连续的气体渗漏点，细菌群落中甲烷氧化菌的比例很高（高达总读取数的2.1％），这使得可以通过对天然样品进行测序来检测它们。在夏季和秋季形成的间歇性气体渗漏点，甲烷营养菌是群落的次要组成部分，只有在甲烷存在下孵育沉积物样品后才能检测到。它们的16S rRNA序列与已知甲烷营养菌的序列之间的低相似性表明，某些生物可能属于该物种和属水平的新分类单元。