It has been reported that Moringa oleifera (MO) has different medicinal properties. The aim of this study was to evaluate the hepatoprotective role of Moringa oleifera extract on acetaminophen-induced liver fibrosis in albino rats on a biochemical and histological basis. Forty male albino rats were divided into four groups: group I (control group), healthy rates; group II (acetaminophen group), rates received acetaminophen for induction of liver fibrosis; group III (treated group), liver fibrosis of rates treated with Moringa oleifera extract; and group IV (prophylactic group), rates treated with Moringa oleifera extract before and after induction of liver fibrosis. Serum liver function parameters were quantified using a spectrophotometer, while tumor necrosis factor α (TNF-α) and transformed growth factor beta (TGF- β) in liver tissue homogenate by means of enzyme-linked immunosorbent assay (ELISA), and expression of liver tissue TNF-α and TGF-genes was measured by real-time PCR after extraction and purification. Hepatic tissue was also evaluated under a microscope for histopathological changes. Our results showed a significant decrease in liver enzymes, TNF-α, and TGF-β in the treated and prophylactic groups compared to the acetaminophen group, and our biochemical data were consistent with the histopathological findings confirming the hepatoprotective effect of Moringa oleifera extract. Biochemical parameters and histopathology results provide evidence that Moringa oleifera ethanolic extract has a great potential to prevent and improve liver damage due to its protective activity.

中文翻译：

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辣木提取物对对乙酰氨基酚诱导的大鼠肝纤维化中TNF-α和TGF-β表达的保肝作用

据报道，辣木 (MO) 具有不同的药用特性。本研究的目的是在生化和组织学基础上评估辣木提取物对对乙酰氨基酚诱导的白化大鼠肝纤维化的保肝作用。四十只雄性白化大鼠分为四组：I组（对照组），健康率；II组（对乙酰氨基酚组），接受对乙酰氨基酚诱导肝纤维化的比率；III组（治疗组），用辣木提取物治疗的肝纤维化率；和组IV（预防组），在诱导肝纤维化之前和之后用辣木提取物治疗的比率。使用分光光度计定量血清肝功能参数，同时通过酶联免疫吸附试验（ELISA）检测肝组织匀浆中肿瘤坏死因子α（TNF-α）和转化生长因子β（TGF-β），并测定肝组织TNF-α和TGF-基因的表达提取纯化后进行实时荧光定量 PCR。还在显微镜下评估肝组织的组织病理学变化。我们的结果显示，与对乙酰氨基酚组相比，治疗组和预防组的肝酶、TNF-α 和 TGF-β 显着降低，我们的生化数据与证实辣木提取物保肝作用的组织病理学结果一致。生化参数和组织病理学结果证明，辣木乙醇提取物由于其保护活性而具有预防和改善肝损伤的巨大潜力。