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The Skeletal Effects of Short-Term Triple Therapy in a Rat Model of Gastric Ulcer Induced by Helicobacter pylori Infection
Drug Design, Development and Therapy ( IF 4.7 ) Pub Date : 2020-12-03 , DOI: 10.2147/dddt.s287239 Sophia Ogechi Ekeuku 1 , Benjamin Ka Seng Thong 1, 2 , Adam Quraisiah 1 , Fazalda Annuar 1 , Alfizah Hanafiah 3 , Mohd Fahami Nur Azlina 1 , Kok-Yong Chin 1
Drug Design, Development and Therapy ( IF 4.7 ) Pub Date : 2020-12-03 , DOI: 10.2147/dddt.s287239 Sophia Ogechi Ekeuku 1 , Benjamin Ka Seng Thong 1, 2 , Adam Quraisiah 1 , Fazalda Annuar 1 , Alfizah Hanafiah 3 , Mohd Fahami Nur Azlina 1 , Kok-Yong Chin 1
Affiliation
Purpose: Triple therapy is the standard therapy to eradicate Helicobacter pylori (H.pylori) infection. Chronic use of proton pump inhibitors (PPIs), a component of triple therapy, is associated with osteoporosis. However, the skeletal effects of short-term triple therapy containing PPI remain elusive. This study aims to determine the skeletal effect of short-term triple therapy in a rat model of gastric ulcer induced by H. pylori.
Methods: Three-month-old male Sprague Dawley rats were assigned to normal control, H. pylori-inoculated group (negative control) and H. pylori-inoculated group receiving triple therapy consisting of omeprazole [2.035 mg/kg body weight (b.w)], amoxicillin (102.80 mg/kg b.w) and clarithromycin (51.37 mg/kg b.w) (n=6/group). H. pylori infection developed for four weeks after inoculation, followed by two-week triple therapy. At the end of the treatment period, femoral bones of the rats were harvested for analysis. Bone mineral density and content of the femurs were determined using dual-energy X-ray absorptiometry, while bone strength was measured with a universal mechanical tester.
Results: Bone mineral content was significantly lower in the negative control group compared to the triple therapy group (p=0.014). Triple therapy decreased strain (vs negative control, p=0.002) and displacement of the femur (vs normal control, p=0.004; vs untreated control, p=0.005). No significant difference was observed in other parameters among the study groups (p> 0.05).
Conclusion: Short-term triple therapy increases bone mineral content but decreases bone strength of rats. Skeletal prophylaxis should be considered for patients on short-term triple therapy containing PPI.
Keywords: bone, gastric ulcer, omeprazole, osteoporosis, osteopenia, proton pump inhibitor
中文翻译：
短期三联疗法对幽门螺杆菌感染大鼠胃溃疡模型的骨骼效应
目的：三联疗法是根除幽门螺杆菌（H.pylori）感染的标准疗法。长期使用质子泵抑制剂 (PPI) 是三联疗法的一个组成部分，与骨质疏松症有关。然而，含有 PPI 的短期三联疗法的骨骼效应仍然难以捉摸。本研究旨在确定短期三联疗法在H. pylori诱导的大鼠胃溃疡模型中的骨骼效应。
方法：将3月龄雄性Sprague Dawley大鼠分为正常对照组、H. pylori接种组（阴性对照组）和H. pylori-接种组接受由奥美拉唑[2.035 mg/kg体重（bw）]、阿莫西林（102.80 mg/kg bw）和克拉霉素（51.37 mg/kg bw）组成的三联疗法（n=6/组）。接种后 4 周出现幽门螺杆菌感染，随后进行为期两周的三联疗法。在治疗期结束时，收获大鼠的股骨用于分析。使用双能 X 射线骨密度仪测定股骨的骨矿物质密度和含量，而使用通用机械测试仪测量骨强度。
结果：与三联疗法组相比，阴性对照组的骨矿物质含量显着降低（p=0.014）。三联疗法降低了应变（与阴性对照相比，p=0.002）和股骨移位（与正常对照相比，p=0.004；与未治疗对照相比，p=0.005）。研究组间其他参数无显着差异（p>0.05）。
结论：短期三联疗法可增加大鼠骨矿物质含量，但降低大鼠骨强度。对于接受含有 PPI 的短期三联疗法的患者，应考虑进行骨骼预防。
关键词：骨，胃溃疡，奥美拉唑，骨质疏松，骨质减少，质子泵抑制剂
更新日期：2020-12-03
Methods: Three-month-old male Sprague Dawley rats were assigned to normal control, H. pylori-inoculated group (negative control) and H. pylori-inoculated group receiving triple therapy consisting of omeprazole [2.035 mg/kg body weight (b.w)], amoxicillin (102.80 mg/kg b.w) and clarithromycin (51.37 mg/kg b.w) (n=6/group). H. pylori infection developed for four weeks after inoculation, followed by two-week triple therapy. At the end of the treatment period, femoral bones of the rats were harvested for analysis. Bone mineral density and content of the femurs were determined using dual-energy X-ray absorptiometry, while bone strength was measured with a universal mechanical tester.
Results: Bone mineral content was significantly lower in the negative control group compared to the triple therapy group (p=0.014). Triple therapy decreased strain (vs negative control, p=0.002) and displacement of the femur (vs normal control, p=0.004; vs untreated control, p=0.005). No significant difference was observed in other parameters among the study groups (p> 0.05).
Conclusion: Short-term triple therapy increases bone mineral content but decreases bone strength of rats. Skeletal prophylaxis should be considered for patients on short-term triple therapy containing PPI.
Keywords: bone, gastric ulcer, omeprazole, osteoporosis, osteopenia, proton pump inhibitor
中文翻译：
短期三联疗法对幽门螺杆菌感染大鼠胃溃疡模型的骨骼效应
目的：三联疗法是根除幽门螺杆菌（H.pylori）感染的标准疗法。长期使用质子泵抑制剂 (PPI) 是三联疗法的一个组成部分，与骨质疏松症有关。然而，含有 PPI 的短期三联疗法的骨骼效应仍然难以捉摸。本研究旨在确定短期三联疗法在H. pylori诱导的大鼠胃溃疡模型中的骨骼效应。
方法：将3月龄雄性Sprague Dawley大鼠分为正常对照组、H. pylori接种组（阴性对照组）和H. pylori-接种组接受由奥美拉唑[2.035 mg/kg体重（bw）]、阿莫西林（102.80 mg/kg bw）和克拉霉素（51.37 mg/kg bw）组成的三联疗法（n=6/组）。接种后 4 周出现幽门螺杆菌感染，随后进行为期两周的三联疗法。在治疗期结束时，收获大鼠的股骨用于分析。使用双能 X 射线骨密度仪测定股骨的骨矿物质密度和含量，而使用通用机械测试仪测量骨强度。
结果：与三联疗法组相比，阴性对照组的骨矿物质含量显着降低（p=0.014）。三联疗法降低了应变（与阴性对照相比，p=0.002）和股骨移位（与正常对照相比，p=0.004；与未治疗对照相比，p=0.005）。研究组间其他参数无显着差异（p>0.05）。
结论：短期三联疗法可增加大鼠骨矿物质含量，但降低大鼠骨强度。对于接受含有 PPI 的短期三联疗法的患者，应考虑进行骨骼预防。
关键词：骨，胃溃疡，奥美拉唑，骨质疏松，骨质减少，质子泵抑制剂
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