In light-sheet fluorescence microscopy (LSFM), using Gaussian beams for light-sheet generation results in a trade-off between the thickness and the field of view (FOV). Here we present a theoretical analysis of using spherical aberration to enlarge the FOV while keeping the light-sheet thickness small. Such spherical aberration can arise when focusing beams through an interface between materials of mismatched refractive indices. The depth-of-focus extension of the Gaussian beam is achieved when using air objectives to focus light into the samples dipped in the immersion medium with a higher refractive index. By scanning this elongated beam, a thin light sheet with a wide FOV can be used for LSFM imaging. Meanwhile, the accompanied sidelobes with the spherical aberrated light sheet, which are mainly distributed in the rear part of the light sheet, are also discussed. Simulation results show that an extended FOV of $64.4\;{\unicode{x00B5}{\rm m}}$ is possible for an objective lens of ${\rm NA} = 0.3$, which is about 5 times that of the unaberrated case. For such an extended FOV, a comparatively thin thickness of $1.38\;{\unicode{x00B5}{\rm m}}$ as well as the first sidelobe about 11.1% of the peak intensity in the center are also demonstrated.

中文翻译：

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在光片荧光显微镜中研究球面像差扩展聚焦能力以扩大视野

在光片荧光显微镜（LSFM）中，使用高斯光束产生光片会在厚度和视场（FOV）之间进行权衡。在这里，我们提供了使用球面像差扩大FOV并同时保持光片厚度较小的理论分析。当将光束聚焦通过折射率不匹配的材料之间的界面时，会出现这种球差。当使用空气物镜将光聚焦到浸入具有较高折射率的浸没介质中的样品中时，可实现高斯光束的聚焦深度扩展。通过扫描该细长光束，可以将具有宽FOV的薄光片用于LSFM成像。同时，带有球形像差的光片的旁瓣主要分布在光片的后部，也进行了讨论。仿真结果表明扩展了FOV对于$ {\ rm NA} = 0.3 $的物镜，可能$ 64.4 \; {\ unicode {x00B5} {\ rm m}} $是$ 53的物镜，约为无差镜情况的5倍。对于这样的扩展FOV，还显示出$ 1.38 \; {\ unicode {x00B5} {\ rm m}} $的相对较薄的厚度以及中心附近峰值强度的11.1％的第一旁瓣。