Objectives: to investigate the expression levels of 1,25(OH)₂D₃ in the peripheral blood from patients with myasthenia gravis (MG) and to correlate levels with retinoid-related orphan receptor γt (RORγt) and forkhead or winged-helix transcription factor 3 (Foxp3) mRNA expression. Methods: Sixty-seven patients with MG were enrolled in the experimental group, and 50 normal subjects were selected as the control group. The expression levels of 1,25(OH)₂D₃ and RORγt and Foxp3 mRNAs were measured in the serum of the 2 patient groups and the relationship between factors were correlated with the severity score of MG. The relationship between the levels of 1,25(OH)₂D₃ and the relative expressions of RORγt and Foxp3 mRNAs was determined. Results: There were no differences between groups regarding patient’s baseline data. 1,25(OH)₂D₃ and RORγt and Foxp3 mRNAs are differentially expressed in the MG group and the control group (p #x3c; 0.05). QMG score is negatively correlated with the expression level of peripheral blood 1,25(OH)₂D₃ and Foxp3 mRNA (r = −0.797, −0.543; p #x3c; 0.01) and positively correlated with the relative expression level of RORγt mRNA (r = 0.539; p #x3c; 0.01). 1,25(OH)₂D₃ expression level was negatively correlated with the relative expression of RORγt mRNA (r = −0.559; p #x3c; 0.01) and positively correlated with the relative expression of Foxp3 mRNA (r = 0.390; p #x3c; 0.01). Conclusions: The levels of 1,25(OH)₂D₃ were shown to be lower in patients with MG compared to normal controls. The observed low levels of 1,25(OH)₂D₃ may lead to changes in the expression of RORγt and Foxp3 mRNAs involved in MG.
Neuroimmunomodulation

中文翻译：

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维生素D3与重症肌无力患者外周血RORγt、Foxp3 mRNA表达的相关性

目的：研究重症肌无力 (MG) 患者外周血中 1,25(OH) ₂ D ₃的表达水平，并将其与维甲酸相关孤儿受体 γt (RORγt) 和叉头或翼螺旋转录相关联因子 3 (Foxp3) mRNA 表达。方法：将67例MG患者纳入实验组，另选50例正常人作为对照组。测定2组患者血清中1,25(OH) ₂ D _{3和RORγt、Foxp3 mRNA的表达水平，并与MG严重程度评分相关因素之间的关系。1,25(OH) 2} D水平之间的关系_图3测定了 RORγt 和 Foxp3 mRNAs 的相对表达量。结果：关于患者的基线数据，各组之间没有差异。1,25(OH) ₂ D ₃和RORγt和Foxp3 mRNA在MG组和对照组中差异表达( p #x3c; 0.05)。QMG评分与外周血1,25(OH) ₂ D ₃和Foxp3 mRNA表达水平呈负相关（r = -0.797, -0.543; p #x3c; 0.01），与RORγt mRNA相对表达水平呈正相关（r = 0.539；p #x3c；0.01）。1,25(OH) ₂ D ₃表达水平与 RORγt mRNA 的相对表达量呈负相关（r = -0.559；p #x3c；0.01），与 Foxp3 mRNA 的相对表达量呈正相关（r = 0.390；p #x3c；0.01）。结论：与正常对照组相比，MG 患者的 1,25(OH) ₂ D _{3水平较低。}观察到的低水平 1,25(OH) ₂ D ₃可能导致与 MG 相关的 RORγt 和 Foxp3 mRNA 的表达发生变化。
神经免疫调节