Ascochyta versabilis is the fungal pathogen that causes the severe leaf spot disease of Pseudostellaria heterophylla (Miq.) Pax, a vital Chinese herbal plant. Here, we deployed PacBio single-molecule real-time long-read sequencing technology to generate a near-complete genome assembly for the A. versabilis KC1 strain and obtained a total of 9.80 Gb raw reads. These reads were processed into a 41.05 Mb genome assembly containing 95 contigs with N50 of 1.70 Mb and a maximum length of 3.93 Mb. A total of 10,457 gene models, of which 1,004 encode putatively secreted proteins, were identified in the genome. This high-quality genome assembly and gene annotation resource will facilitate the institution of functional genetic studies aimed at providing a better insight into the infection mechanisms of A. versabilis to support the development of effective control strategies for leaf spot disease of P. heterophylla.

Ascochyta versabilis是一种真菌病原体，可导致一种重要的中草药植物——Pseudostellaria heterophylla（Miq。）Pax的严重叶斑病。在这里，我们部署了PacBio单分子实时长读测序技术，以产生通用的拟南芥基因组装配体KC1株，并获得了总计9.80 Gb的原始读数。将这些读段处理成41.05 Mb基因组，其中包含95个重叠群，N50为1.70 Mb，最大长度为3.93 Mb。在基因组中鉴定出总共10,457个基因模型，其中1,004个编码假定分泌的蛋白质。这种高质量的基因组装配和基因注释资源将有助于开展功能性遗传研究，旨在更好地了解无花农杆菌的感染机制，以支持对异叶假单胞菌叶斑病的有效控制策略的开发。