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Automated device for continuous stirring while sampling in liquid chromatography systems
Communications Chemistry ( IF 5.9 ) Pub Date : 2020-12-03 , DOI: 10.1038/s42004-020-00427-5
Omer Markovitch 1, 2 , Jim Ottelé 2 , Obe Veldman 3 , Sijbren Otto 2
Affiliation  

Ultra-performance liquid chromatography is a common analysis tool, and stirring is common in many laboratory setups. Here we show a device which enables continuous stirring of samples whilst inside an ultra-performance liquid chromatography system. Utilizing standard magnetic stirring bars that fit standard vials, the device allows for the automation of experimental setups that require stirring. The device is designed such that it can replace the standard sample holder and fits in its place, while being battery operated. The use of three-dimensional (3D) printing and commercially available parts enables low-effort and low-cost device production, as well as easy modifications. Testing the device was performed by video analysis and by following the kinetics of a dynamic combinatorial library that is known to be exquisitely sensitive to agitation, as a result of involving a fiber growth-breakage mechanism. Design files and schematics are provided.



中文翻译:

在液相色谱系统中取样时连续搅拌的自动装置

超高效液相色谱是一种常见的分析工具,搅拌在许多实验室设置中很常见。在这里,我们展示了一种能够在超高效液相色谱系统内连续搅拌样品的装置。该设备使用适合标准小瓶的标准磁力搅拌棒,可实现需要搅拌的实验设置的自动化。该设备的设计使其可以更换标准样品架并安装到位,同时由电池供电。使用三维 (3D) 打印和市售部件可实现低工作量和低成本的设备生产,以及易于修改。该设备的测试是通过视频分析和遵循动态组合库的动力学进行的,该库已知对搅动非常敏感,由于涉及纤维生长断裂机制。提供设计文件和原理图。

更新日期:2020-12-03
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