ABSTRACT

We report the formulation of nanoassemblies (NAs) comprising C225 conjugates Au-PFH-NAs (C-Au-PFH-NAs) for low-intensity focused ultrasound diagnosis ablation of thyroid cancer. C-Au-PFH-NAs showed excellent stability in water, phosphate-buffered saline (PBS), and 20% rat serum. Transmission electron microscopy (TEM) images also revealed the effective construction of C-Au-PFH-NAs as common spherical assemblies. The incubation of C625 thyroid carcinoma with C-Au-PFH-NAs triggers apoptosis, as confirmed by flow cytometry analysis. The C-Au-PFH-NAs exhibited antitumour efficacy in human thyroid carcinoma xenografts, where histopathological results further confirmed these outcomes. Furthermore, we were able to use low-intensity focused ultrasound diagnosis imaging (LIFUS) to examine the efficiency of C-Au-PFH-NAs in thyroid carcinoma in vivo. These findings clearly show that the use of LIFUS agents with high-performance imaging in different therapeutic settings will have extensive potential for future biomedical applications.

摘要

我们报告了包含C225偶联物Au-PFH-NAs（C-Au-PFH-NAs）的纳米组件（NA）的配方，用于甲状腺癌的低强度聚焦超声诊断消融。C-Au-PFH-NAs在水，磷酸盐缓冲液（PBS）和20％大鼠血清中显示出极好的稳定性。透射电子显微镜（TEM）图像还揭示了C-Au-PFH-NA作为常见球形组件的有效构造。流式细胞仪分析证实，将C625甲状腺癌与C-Au-PFH-NAs一起孵育可触发凋亡。C-Au-PFH-NAs在人甲状腺癌异种移植物中显示出抗肿瘤功效，其中组织病理学结果进一步证实了这些结果。此外，我们能够使用低强度聚焦超声诊断成像（LIFUS）来检查C-Au-PFH-NAs在甲状腺癌中的有效性体内。这些发现清楚地表明，在不同的治疗环境中使用具有高性能成像功能的LIFUS试剂在未来的生物医学应用中将具有广阔的潜力。