It is now well known that the quorum sensing (QS) mechanism coordinates the production of several virulence factors and biofilm formation in most pathogenic microorganisms. Aeromonas hydrophila is a prime pathogen responsible for frequent outbreaks in aquaculture settings. Recent studies have also continuously reported that A. hydrophila regulates virulence factor production and biofilm formation through the QS system. In addition to the presence of antibiotic resistance genes, biofilm-mediated antibiotic resistance increases the severity of A. hydrophila infections. To control the bacterial pathogenesis and subsequent infections, targeting the QS mechanism has become one of the best alternative methods. Though very few compounds were identified as QS inhibitors against A. hydrophila, to date, the screening and identification of new and effective natural QS inhibitors is a dire necessity to control the infectious A. hydrophila. The present study endorses naringin (NA) as an anti-QS and anti-infective agent against A. hydrophila. Initially, the NA showed a concentration-dependent biofilm reduction against A. hydrophila. Furthermore, the results of microscopic analyses and quantitative virulence assays displayed the promise of NA as a potential anti-QS agent. Subsequently, the downregulation of ahh1, aerA, lip and ahyB validate the interference of NA in virulence gene expression. Furthermore, the in vivo assays were carried out in zebrafish model system to evaluate the anti-infective potential of NA. The outcome of the immersion challenge assay showed that the recovery rate of the zebrafish has substantially increased upon treatment with NA. Furthermore, the quantification of the bacterial load upon NA treatment showed a decreased level of bacterial counts in zebrafish when compared to the untreated control. Moreover, the NA treatment averts the pathogen-induced histoarchitecture damages in vital organs of zebrafish, compared to their respective controls. The current study has thus analyzed the anti-QS and anti-infective capabilities of NA and could be employed to formulate effective treatment measures against A. hydrophila infections.

现在众所周知，群体感应（QS）机制协调了大多数致病微生物中几种毒力因子的产生和生物膜的形成。 嗜水气单胞菌是导致水产养殖频繁爆发的主要病原体。最近的研究也不断报告亲水曲霉通过QS系统调节毒力因子的产生和生物膜的形成。除了存在抗生素抗性基因外，生物膜介导的抗生素抗性还会增加亲水曲霉感染。为了控制细菌的发病机理和随后的感染，靶向QS机制已成为最好的替代方法之一。虽然很少有化合物被鉴定为QS抑制剂亲水曲霉迄今为止，筛选和鉴定新型有效的天然QS抑制剂是控制传染病的迫切需要。 亲水曲霉。本研究赞同柚皮苷（NA）作为抗QS和抗感染药亲水曲霉。最初，NA显示出针对浓度的生物膜减少亲水曲霉。此外，显微分析和定量毒力测定的结果显示了NA作为潜在的抗QS药物的前景。随后，下调了ahh1， 航空， 唇 和 乙醛验证NA对毒力基因表达的干扰。此外，体内实验在斑马鱼模型系统中进行，以评估NA的抗感染潜力。浸没攻击试验的结果表明，用NA处理后，斑马鱼的回收率已大大提高。此外，与未处理的对照相比，NA处理后细菌载量的定量显示斑马鱼中细菌数量的减少。此外，与它们各自的对照相比，NA处理可避免斑马鱼重要器官中病原体引起的组织结构损害。因此，本研究分析了NA的抗QS和抗感染能力，可用于制定针对NA的有效治疗措施。亲水曲霉 感染。