Banana is a key staple food and fruit in countries all over the world. However, the development of the global banana industry is seriously threatened by Fusarium wilt disease, which is caused by Fusarium oxysporum f. sp. cubense (Foc). In particular, Foc tropical race 4 (Foc TR4) could infect more than 80% of global banana and plantain crops. Until now, there were no commercial chemicals or resistant cultivars available to control the disease. Biological control using actinomycetes is considered a promising strategy. In this study, 88 actinomycetes were isolated from a banana orchard without symptoms of Fusarium wilt disease for more than 10 years. An actinobacterial strain labeled as JBS5-6 has exhibited strong antifungal activities against Foc TR4 and other selected 10 phytopathogenic fungi. Based on phenotypic and biochemical traits as well as complete genome analysis, strain JBS5-6 was assigned to Streptomyces violaceusniger. Extracts of the strain inhibited the mycelial growth and spore germination of Foc TR4 by destroying membrane integrity and the ultrastructure of cells. The complete genome of strain JBS5-6 was sequenced and revealed a number of key function gene clusters that contribute to the biosynthesis of active secondary metabolites. Sixteen chemical compounds were further identified by gas chromatography-mass spectrometry (GC-MS). 5-hydroxymethyl-2-furancarboxaldehyde was one of the dominant components in strain JBS5-6 extracts. Moreover, fermentation broth of strain JBS5-6 significantly reduced the disease index of banana seedlings by inhibiting the infection of Foc TR4 in a pot experiment. Hence, strain JBS5-6 is a potential biocontrol agent for the management of disease and the exploitation of biofertilizer.

香蕉是世界各国的主要主食和水果。然而，全球香蕉产业的发展正受到由镰刀菌引起的枯萎病的严重威胁。尖孢镰刀菌F。sp。库本塞（焦点）。特别是，Foc 热带小种 4 (Foc TR4) 可能感染全球 80% 以上的香蕉和大蕉作物。到目前为止，还没有商业化学品或抗性品种可用于控制这种疾病。使用放线菌的生物防治被认为是一种有前途的策略。这项研究从一个香蕉园中分离出 88 种放线菌，这些放线菌在 10 多年的时间里都没有出现镰刀菌枯萎病的症状。标记为 JBS5-6 的放线菌菌株对 Foc TR4 和其他选定的 10 种植物病原真菌表现出强大的抗真菌活性。根据表型和生化特征以及完整的基因组分析，菌株 JBS5-6 被分配为黑色紫罗兰链霉菌。该菌株的提取物通过破坏细胞膜的完整性和超微结构来抑制 Foc TR4 的菌丝生长和孢子萌发。对菌株 JBS5-6 的完整基因组进行了测序，揭示了许多有助于活性次级代谢产物生物合成的关键功能基因簇。通过气相色谱-质谱法 (GC-MS) 进一步鉴定了 16 种化合物。5-羟甲基-2-呋喃甲醛是菌株JBS5-6提取物中的主要成分之一。此外，在盆栽试验中，菌株JBS5-6的发酵液通过抑制Foc TR4的侵染，显着降低了香蕉幼苗的发病指数。因此，菌株 JBS5-6 是用于疾病管理和生物肥料开发的潜在生物防治剂。