Glycan moieties in Entamoeba histolytica ubiquitin are immunodominant,Parasite Immunology

当前位置： X-MOL 学术 › Parasite Immunol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Glycan moieties in Entamoeba histolytica ubiquitin are immunodominant
Parasite Immunology ( IF 1.4 ) Pub Date : 2020-12-03 , DOI: 10.1111/pim.12812
María S Flores ₁ , Adriana Obregón-Cardenas ₁ , Roberto Rangel ₁ , Eva Tamez ₁ , Andrés Flores ₁ , Laura Trejo-Avila ₁ , Isela Quintero ₁ , Katiushka Arévalo ₁ , María G Maldonado ₁ , Fátima L Gandarilla ₁ , Luis Galán ₁

Affiliation

The ubiquitin‐proteasome system plays a central role performing several functions to maintain parasite homeostasis. We have reported the partial characterization of N‐linked glycosylation profile in E. histolytica ubiquitin (EhUb). Here we examined the immunogenicity and antigenicity of carbohydrates in EhUbiquitin. Rabbits were immunized with purified EhUbiquitin or purified recombinant rUb expressed by E. coli. Using Western Blot, we explored the immunogenicity and antigenicity of protein portion and carbohydrates moiety. Interestingly, immunized rabbits produced antibodies to both Ub glycoprotein and rUb; but antibodies against carbohydrates were immunodominant, rather than antibodies to the protein moiety of EhUbiquitin. In addition, we observed that antibodies to protein moiety are not conserved in serum unless antigen is continually administrated. Conversely, anti‐Ub glycoprotein antibodies are well maintained in circulation. In humans, infection with Entamoeba histolytica induces strong IgG anti‐Ub response. The human antibodies recognize both, the protein moieties and the glycosylated structure. Entamoeba histolytica ubiquitin is immunogenic and antigenic. The glycan moieties are immunodominant and induces IgG. These data open the door to use carbohydrates as potential targets for diagnose tests, drugs and vaccine to prevent this parasitic disease.

中文翻译：

溶组织内阿米巴泛素中的聚糖部分是免疫显性的

泛素-蛋白酶体系统在执行多种功能以维持寄生虫体内平衡方面发挥着核心作用。我们已经报告了溶组织大肠杆菌泛素 (EhUb) 中 N 联糖基化谱的部分表征。在这里，我们检查了 EhUbiquitin 中碳水化合物的免疫原性和抗原性。用纯化的 EhUbiquitin 或由大肠杆菌表达的纯化重组 rUb 免疫兔. 使用蛋白质印迹，我们探索了蛋白质部分和碳水化合物部分的免疫原性和抗原性。有趣的是，免疫兔产生了针对 Ub 糖蛋白和 rUb 的抗体。但是针对碳水化合物的抗体是免疫显性的，而不是针对 EhUbiquitin 蛋白质部分的抗体。此外，我们观察到，除非连续施用抗原，否则针对蛋白质部分的抗体在血清中是不保守的。相反，抗 Ub 糖蛋白抗体在循环中保持良好。在人类中，溶组织内阿米巴感染会诱导强烈的 IgG 抗 Ub 反应。人抗体识别蛋白质部分和糖基化结构。溶组织内阿米巴泛素具有免疫原性和抗原性。聚糖部分是免疫显性的并诱导 IgG。

更新日期：2020-12-03

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11