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Dentin erosion preventive effects of various plant extracts: An in vitro atomic force microscopy, scanning electron microscopy, and nanoindentation study
Microscopy Research and Technique ( IF 2.5 ) Pub Date : 2020-12-02 , DOI: 10.1002/jemt.23665
Ayca Sarialioglu Gungor 1 , Nazmiye Donmez 1
Affiliation  

The overall consumption of acidic beverages has become more common, making the prevention and treatment of dental erosion an important consideration. The aim of this in vitro study was to evaluate the effect of various plant extracts in preventing dentin erosion. Seven experimental groups (fluoride‐free water, fluoride‐containing mouthwash [Colgate Plax], green tea, rosehip, clove, pomegranate, and grape seed) were formed, each consisting of 20 bovine dentin samples. The specimens were exposed daily to demineralization and remineralization cycles three times per day over 5 days through a 5‐min plant extract application before each erosive episode. Surface roughness, nanohardness values, and morphological changes on dentin surfaces were examined using atomic force microscopy (AFM), nanoindentation, and scanning electron microscopy (SEM). The data were subjected to Kruskal–Wallis and Friedman tests (p < .05). There were statistically significant differences between the groups in terms of nanohardness values (p < .05), except for the pomegranate and grape seed groups. The highest nanohardness value was observed in the clove group (1.24 ± 0.34 GPa), whereas the lowest nanohardness value was noted in the grape seed group (0.20 ± 0.04 GPa). The nanohardness values of positive and negative control groups after erosion cycles were statistically higher than the initial nanohardness values (p < .05).There was no statistically significant difference between the groups in surface roughness values (p > .05). Macromolecular deposits were observed both in the SEM and AFM images of the pomegranate, Colgate, and rosehip groups. It can be concluded that the clove extract group is more successful in preventing dentin erosion than the other groups. Green tea is also effective in preventing dentin erosion, similar to clove extract.

中文翻译:

各种植物提取物的牙本质侵蚀预防作用:体外原子力显微镜、扫描电子显微镜和纳米压痕研究

酸性饮料的整体消费变得更加普遍,使得预防和治疗牙齿侵蚀成为重要的考虑因素。这项体外研究的目的是评估各种植物提取物在预防牙本质侵蚀方面的作用。形成七个实验组(无氟水、含氟漱口水 [Colgate Plax]、绿茶、玫瑰果、丁香、石榴和葡萄籽),每组由 20 个牛牙本质样品组成。在每次侵蚀发作之前,通过 5 分钟的植物提取物应用,在 5 天内,每天将样本暴露于脱矿和再矿化循环中 3 次。使用原子力显微镜 (AFM)、纳米压痕和扫描电子显微镜 (SEM) 检查牙本质表面的表面粗糙度、纳米硬度值和形态变化。p < .05)。除了石榴和葡萄籽组外,各组之间在纳米硬度值方面存在统计学显着差异 ( p < .05)。丁香组的纳米硬度值最高 (1.24 ± 0.34 GPa),而葡萄籽组的纳米硬度值最低 (0.20 ± 0.04 GPa)。侵蚀循环后阳性和阴性对照组的纳米硬度值在统计学上高于初始纳米硬度值(p < .05)。各组之间的表面粗糙度值无统计学差异(p> .05)。在石榴、高露洁和玫瑰果组的 SEM 和 AFM 图像中均观察到大分子沉积物。可以得出结论,丁香提取物组在防止牙本质侵蚀方面比其他组更成功。绿茶也能有效防止牙本质侵蚀,类似于丁香提取物。
更新日期:2020-12-02
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