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Pathway‐Based Approaches for Assessing Biological Hazards of Complex Mixtures of Contaminants: A Case Study in the Maumee River
Environmental Toxicology and Chemistry ( IF 3.6 ) Pub Date : 2020-12-03 , DOI: 10.1002/etc.4949
G T Ankley 1 , J P Berninger 1 , B R Blackwell 1 , J E Cavallin 1 , T W Collette 2 , D R Ekman 2 , K A Fay 1 , D J Feifarek 1 , K M Jensen 1 , M D Kahl 1 , J D Mosley 2 , S T Poole 1 , E C Randolph 1 , D Rearick 3 , A L Schroeder 4 , J Swintek 5 , D L Villeneuve 1
Affiliation  

Assessment of ecological risks of chemicals in the field usually involves complex mixtures of known and unknown compounds. We describe the use of pathway‐based chemical and biological approaches to assess the risk of chemical mixtures in the Maumee River (OH, USA), which receives a variety of agricultural and urban inputs. Fathead minnows (Pimephales promelas) were deployed in cages for 4 d at a gradient of sites along the river and adjoining tributaries in 2012 and during 2 periods (April and June) in 2016, in conjunction with an automated system to collect composite water samples. More than 100 industrial chemicals, pharmaceuticals, and pesticides were detected in water at some of the study sites, with the greatest number typically found near domestic wastewater treatment plants. In 2016, there was an increase in concentrations of several herbicides from April to June at upstream agricultural sites. A comparison of chemical concentrations in site water with single chemical data from vitro high‐throughput screening (HTS) assays suggested the potential for perturbation of multiple biological pathways, including several associated with induction or inhibition of different cytochrome P450 (CYP) isozymes. This was consistent with direct effects of water extracts in an HTS assay and induction of hepatic CYPs in caged fish. Targeted in vitro assays and measurements in the caged fish suggested minimal effects on endocrine function (e.g., estrogenicity). A nontargeted mass spectroscopy‐based analysis suggested that hepatic endogenous metabolite profiles in caged fish covaried strongly with the occurrence of pesticides and pesticide degradates. These studies demonstrate the application of an integrated suite of measurements to help understand the effects of complex chemical mixtures in the field. Environ Toxicol Chem 2021;40:1098–1122. © 2020 SETAC. This article has been contributed to by US Government employees and their work is in the public domain in the USA.

中文翻译:

评估复杂污染物混合物的生物危害的基于路径的方法:莫米河的案例研究

现场化学品的生态风险评估通常涉及已知和未知化合物的复杂混合物。我们描述了使用基于路径的化学和生物方法来评估莫米河(俄亥俄州,美国)中化学混合物的风险,该河接受各种农业和城市投入。黑头鲦鱼 ( Pimephales promelas) 于 2012 年和 2016 年的 2 个时期(4 月和 6 月)在沿河和毗邻支流的梯度地点部署在笼子中 4 天,并结合自动化系统收集复合水样。在一些研究地点的水中检测到 100 多种工业化学品、药物和杀虫剂,其中数量最多的通常是生活污水处理厂附近。2016 年,4 月至 6 月上游农业场地的几种除草剂浓度有所增加。现场水中的化学浓度与来自体外高通量筛选 (HTS) 测定的单一化学数据的比较表明,可能会扰乱多种生物途径,包括与诱导或抑制不同细胞色素 P450 (CYP) 同工酶相关的几种途径。这与 HTS 测定中水提取物的直接作用和笼养鱼中肝 CYP 的诱导一致。在笼养鱼中进行的靶向体外测定和测量表明对内分泌功能的影响很小(例如,雌激素)。基于非靶向质谱的分析表明,笼养鱼的肝脏内源性代谢物谱与农药和农药降解物的发生密切相关。这些研究展示了一套集成测量的应用,以帮助了解复杂化学混合物在现场的影响。基于非靶向质谱的分析表明,笼养鱼的肝脏内源性代谢物谱与农药和农药降解物的发生密切相关。这些研究展示了一套集成测量的应用,以帮助了解复杂化学混合物在现场的影响。基于非靶向质谱的分析表明,笼养鱼的肝脏内源性代谢物谱与农药和农药降解物的发生密切相关。这些研究展示了一套集成测量的应用,以帮助了解复杂化学混合物在现场的影响。环境毒物化学2021;40:1098–1122。© 2020 SETAC。本文由美国政府雇员贡献,他们的工作在美国属于公共领域。
更新日期:2020-12-03
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