Abstract The global regulator LaeA has been confirmed to govern the production of secondary metabolites in fungi. Herein, we examined the role of LaeA in Chaetomium globosum. Similarly as observed in other filamentous, CgLaeA had a significant effect on the secondary metabolism. The ΔCglaeA mutant strain did not exhibit chaetoglobosin A, whereas its production was restored in the CglaeAC strain. In addition, CglaeA overexpression led to an increase in chaetoglobosin A production. Transcriptional examination of the mutants indicated that CgLaeA positively regulated the expression of pathway-specific transcription factor CgcheR, while another global regulator CgvelB was negatively regulated by CgLaeA. Furthermore, CgLaeA also affected the morphological phenotypes of fungi. The ΔCglaeA mutant strains exhibited decreased sporulation and pigmentation compared with the wild-type strain, whereas the phenotypes were restored in the CglaeAC strain. Moreover, OE::CglaeA exhibited increased levels of sporulation and pigmentation. Moreover, inhibition activity against phytopathogenic fungi affected by decreased mycotoxin production of the ΔCglaeA mutant strain.

中文翻译：

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LaeA 对球毛壳孢子形成、色素沉着和次生代谢的需求

摘要 全球调节器 LaeA 已被证实控制真菌中次级代谢物的产生。在此，我们研究了 LaeA 在球毛壳菌中的作用。与在其他丝状体中观察到的类似，CgLaeA 对次级代谢有显着影响。ΔCglaeA 突变株没有表现出毛壳球蛋白 A，而在 CglaeAC 株中恢复了它的生产。此外，CglaeA 过表达导致毛壳球蛋白 A 产量增加。突变体的转录检查表明 CgLaeA 正调节通路特异性转录因子 CgcheR 的表达，而另一个全局调节因子 CgvelB 受 CgLaeA 负调节。此外，CgLaeA 还影响真菌的形态表型。与野生型菌株相比，ΔCglaeA 突变菌株的孢子形成和色素沉着减少，而 CglaeAC 菌株的表型恢复。此外，OE::CglaeA 表现出更高水平的孢子形成和色素沉着。此外，对植物病原真菌的抑制活性受到 ΔCglaeA 突变株霉菌毒素产量降低的影响。