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A highly sensitive flow injection amperometric glucose biosensor using a gold nanoparticles/polytyramine/Prussian blue modified screen-printed carbon electrode
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2020-12-03 , DOI: 10.1016/j.bioelechem.2020.107718
Suntisak Khumngern 1 , Ratchaneekorn Jirakunakorn 1 , Panote Thavarungkul 1 , Proespichaya Kanatharana 1 , Apon Numnuam 1
Affiliation  

A novel oxidase enzyme sensor was fabricated based on the chemisorption of highly active glucose oxidase (GOx) on gold nanoparticles that were adsorbed on a polytyramine layer (AuNPs/Pty). The GOx/AuNPs/Pty assembly was coated on a Prussian blue (PB)-modified screen-printed carbon electrode (SPCE) to produce the GOx/AuNPs/Pty/PB/SPCE biosensor. The amperometric glucose biosensor response was measured at −0.10 V using a Ag pseudo-reference electrode through the reduction current of the PB mediator in a flow injection analysis system. Under optimised experimental conditions, the developed biosensor displayed linearity over the 1.0 μM–1.0 mM glucose concentration range and a limit of detection of 1.0 μM (S/N ≥ 3). A low value for the Michaelis constant of 0.21 mM indicated that the immobilised GOx had high affinity for glucose. The developed biosensor exhibited excellent operational stability over 374 injections, long-term stability over 3 weeks, good reproducibility (relative standard deviations = 1.9%–4.3%) and high selectivity. The results obtained analysing glucose in blood plasma samples were satisfactory when compared with the corresponding results recorded implementing the standard hexokinase-spectrophotometric method (P > 0.05).



中文翻译:

使用金纳米颗粒/聚酪胺/普鲁士蓝修饰的丝网印刷碳电极的高灵敏流动注射电流型葡萄糖生物传感器

基于高活性葡萄糖氧化酶(GOx)在金纳米颗粒上的化学吸附,制造了一种新型的氧化酶传感器,金纳米颗粒吸附在聚酪胺层(AuNPs / Pty)上。将GOx / AuNPs / Pty组件涂覆在普鲁士蓝(PB)修饰的丝网印刷碳电极(SPCE)上,以生产GOx / AuNPs / Pty / PB / SPCE生物传感器。在流动注射分析系统中,使用Ag伪参比电极通过PB介体的还原电流在-0.10 V下测量了安培型葡萄糖生物传感器响应。在优化的实验条件下,开发的生物传感器在1.0 μM–1.0 mM葡萄糖浓度范围内显示线性,检测限为1.0μM(S / N≥3)。Michaelis常数的低值是0.21mM,表明固定化的GOx对葡萄糖具有高亲和力。先进的生物传感器在374次进样中表现出出色的操作稳定性,在3周内具有长期稳定性,重现性好(相对标准偏差= 1.9%–4.3%)和高选择性。与采用标准己糖激酶分光光度法记录的相应结果相比,分析血浆样品中的葡萄糖所获得的结果令人满意(P  > 0.05)。

更新日期:2020-12-15
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