A novel oxidase enzyme sensor was fabricated based on the chemisorption of highly active glucose oxidase (GOx) on gold nanoparticles that were adsorbed on a polytyramine layer (AuNPs/Pty). The GOx/AuNPs/Pty assembly was coated on a Prussian blue (PB)-modified screen-printed carbon electrode (SPCE) to produce the GOx/AuNPs/Pty/PB/SPCE biosensor. The amperometric glucose biosensor response was measured at −0.10 V using a Ag pseudo-reference electrode through the reduction current of the PB mediator in a flow injection analysis system. Under optimised experimental conditions, the developed biosensor displayed linearity over the 1.0 μM–1.0 mM glucose concentration range and a limit of detection of 1.0 μM (S/N ≥ 3). A low value for the Michaelis constant of 0.21 mM indicated that the immobilised GOx had high affinity for glucose. The developed biosensor exhibited excellent operational stability over 374 injections, long-term stability over 3 weeks, good reproducibility (relative standard deviations = 1.9%–4.3%) and high selectivity. The results obtained analysing glucose in blood plasma samples were satisfactory when compared with the corresponding results recorded implementing the standard hexokinase-spectrophotometric method (P > 0.05).

基于高活性葡萄糖氧化酶（GOx）在金纳米颗粒上的化学吸附，制造了一种新型的氧化酶传感器，金纳米颗粒吸附在聚酪胺层（AuNPs / Pty）上。将GOx / AuNPs / Pty组件涂覆在普鲁士蓝（PB）修饰的丝网印刷碳电极（SPCE）上，以生产GOx / AuNPs / Pty / PB / SPCE生物传感器。在流动注射分析系统中，使用Ag伪参比电极通过PB介体的还原电流在-0.10 V下测量了安培型葡萄糖生物传感器响应。在优化的实验条件下，开发的生物传感器在1.0 μM–1.0 mM葡萄糖浓度范围内显示线性，检测限为1.0μM（S / N≥3）。Michaelis常数的低值是0.21mM，表明固定化的GOx对葡萄糖具有高亲和力。先进的生物传感器在374次进样中表现出出色的操作稳定性，在3周内具有长期稳定性，重现性好（相对标准偏差= 1.9％–4.3％）和高选择性。与采用标准己糖激酶分光光度法记录的相应结果相比，分析血浆样品中的葡萄糖所获得的结果令人满意（P  > 0.05）。