The immunomodulatory properties of immunobiological drugs Glutoxim and Phosprenyl we well as vesicular stomatitis virus and inactivated tick-borne encephalitis vaccine virus were studied using human diploid fibroblast cell line from the collection of M. P. Chumakov Federal Research Center for Research and Development of Immunobiological Products. All tested preparations exhibited immunomodulatory activity in human diploid fibroblast cell line. Glutoxim in doses of 0.1 and 0.25 μg/ml stimulated production of IL-6 and IL-10 during 24-48 h of culturing, but did not stimulate production of IL-1β. Phosprenyl, on the contrary, increased production of IL-1β and the levels of IL-6 and IL-10. Vesicular stomatitis virus stimulated the production of IL-1β, IL-6, and IL-10, while inactivated tick-borne encephalitis vaccine virus stimulated the production of cytokines IL-8 and IL-18. Immunomodulatory activity of inactivated tick-borne encephalitis vaccine virus was first demonstrated in the in vitro system.

中文翻译：

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人二倍体成纤维细胞系——研究现代免疫生物学药物和病毒免疫调节特性的模型系统

使用来自 MP Chumakov 联邦免疫生物产品研究与开发研究中心收集的人二倍体成纤维细胞系，研究了免疫生物药物谷氨酰胺和磷烯基以及水泡性口炎病毒和灭活的蜱传脑炎疫苗病毒的免疫调节特性。所有测试的制剂在人二倍体成纤维细胞系中表现出免疫调节活性。0.1 和 0.25 μg/ml 剂量的谷氨酰胺在培养 24-48 小时期间刺激 IL-6 和 IL-10 的产生，但不刺激 IL-1β 的产生。相反，Phosprenyl 增加了 IL-1β 的产生以及 IL-6 和 IL-10 的水平。水泡性口炎病毒刺激了 IL-1β、IL-6 和 IL-10 的产生，而灭活的蜱传脑炎疫苗病毒会刺激细胞因子 IL-8 和 IL-18 的产生。灭活的蜱传脑炎疫苗病毒的免疫调节活性首次在体外系统中得到证实。