Periplanetasin-2 from cockroach exhibits broad spectrum of antimicrobial activity. The underlying antibacterial mechanisms rely on the stimulation of reactive oxygen species overproduction to induce apoptotic cell death. A promising strategy to increase the bioavailability of periplanetasin-2 involves reducing the dose through combination therapy with other antibacterials, that show synergistic effects. Thus, the synergistic antibacterial activity of periplanetasin-2 with conventional antibacterial agents and its mechanisms was examined against Escherichia coli in this study. Among the agents tested, the combinations of periplanetasin-2 with vancomycin and chloramphenicol exhibited synergistic effects. Periplanetasin-2 in combination with vancomycin and chloramphenicol demonstrated antibacterial activity through the intracellular oxidative stress response. The combination with vancomycin resulted in the enhancement of bacterial apoptosis-like death, whereas the combination with chloramphenicol enhanced oxidative stress damage. These synergistic interactions of periplanetasin-2 can help broaden the spectrum of conventional antibiotics. The combination of antimicrobial peptides and conventional antibiotics is proposed as a novel perspective of treatment to combat severe bacterial infection.

中文翻译：

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Periplanetasin-2 增强万古霉素或氯霉素对大肠杆菌的抗菌性能。

来自蟑螂的 Periplanetasin-2 表现出广谱的抗菌活性。潜在的抗菌机制依赖于刺激活性氧过量产生以诱导凋亡细胞死亡。一种提高 periplanetasin-2 生物利用度的有前途的策略包括通过与其他抗菌药物联合治疗来减少剂量，这显示出协同作用。因此，本研究考察了 periplanetasin-2 与常规抗菌剂的协同抗菌活性及其机制对大肠杆菌的作用。在测试的药物中，periplanetasin-2 与万古霉素和氯霉素的组合表现出协同作用。Periplanetasin-2 联合万古霉素和氯霉素通过细胞内氧化应激反应表现出抗菌活性。与万古霉素的组合导致细菌凋亡样死亡的增强，而与氯霉素的组合增强氧化应激损伤。periplanetasin-2 的这些协同相互作用可以帮助拓宽常规抗生素的范围。抗菌肽和常规抗生素的组合被提议作为治疗严重细菌感染的新视角。periplanetasin-2 的这些协同相互作用可以帮助拓宽常规抗生素的范围。抗菌肽和常规抗生素的组合被提议作为治疗严重细菌感染的新视角。periplanetasin-2 的这些协同相互作用可以帮助拓宽常规抗生素的范围。抗菌肽和常规抗生素的组合被提议作为治疗严重细菌感染的新视角。