We have studied the effects of CuO nanoparticles with the mean lateral dimension 300500 nm and thickness about 50 nm on hybrid poplaraspen clones at various stages of micropropagation. It has been established that CuO nanoparticles display a strong sterilizing activity and enhance rhizogenesis in microplants. At the culture establishment stage exposure to 1.5 - 3 μg/L CuO increased the number of leaves and roots, and stimulated the root system development. At 15 μg/L had an inhibiting effect on the studied parameters. At the multiplication stage we observed a considerable decrease in the height of shoots, and thickness of leaf lamina and stem diameter. At the same time a significant increase in the regenerants survival rate was observed (+20%), together with improved rhizogenesis. During the rooting stage, a 30% increase in the number of microclones with roots was detected, as well as a twofold increase in the number of roots on each plant. Besides, the nanomaterial had a stimulating effect on the shoots growth, increasing their height by 25%.Thus, it has been established that CuO nanoparticles produce differently directed effects on the growth and development of poplaraspen hybrid microclones, depending on the micropropagation stage.

我们研究了平均横向尺寸为 300500 nm、厚度约为 50 nm 的 CuO 纳米粒子在微繁殖的各个阶段对杂化白杨无性系的影响。已经确定，CuO 纳米颗粒在微型植物中表现出强烈的杀菌活性并增强根际发生。在培养建立阶段，暴露于 1.5 - 3 μg/L CuO 增加了叶和根的数量，并刺激了根系的发育。15 μg/L 对研究参数有抑制作用。在繁殖阶段，我们观察到枝条高度、叶片厚度和茎直径显着降低。同时，观察到再生体存活率显着增加（+20%），同时根际发生改善。在生根阶段，检测到有根的微克隆数量增加了 30%，每株植物的根数增加了两倍。此外，纳米材料对新芽的生长有刺激作用，使枝高增加了25%。因此，已经确定CuO纳米粒子对杨树杂化微克隆的生长和发育产生不同的定向作用，这取决于微繁殖阶段。