We have studied the impact of graphene oxide (GO) at 1.5 – 15 μg/L on Betula pubescens microclones. Discovered, that the nanomaterial protects the plants against phytopathogens at the stage of culture establishment and promotes a 25% increase in the shoots survival rate. Further growth stimulation was observed in the groups treated with 1.5 and 3 μg/L, where the shoots length increased by 1.8 and 1 cm, and the number of leaves increased by 2 and 1.5 times respectively. 15 μg/L reduced the height of shoots by 20%. At the multiplication stage addition of GO enhanced the plantlets survival rate by 20% and had a stimulating effect on the birch regenerants growth, as well as on the number of leaves. The data on the plantlets survival rate and their morphometric analysis results correspond well with the biochemical assessment, namely in the GO group both photosynthetic and catalase activity exceeded those of the control group by 2 and 1.5 times, respectively. At the same time, histological analysis exhibits reduced number of stomata in the GO-treated group. At the rooting stage addition of 1.5 μg/L GO to the growth medium increased the number of plantlets with roots by 10% compared to control.

我们研究了 1.5 – 15 μg/L 的氧化石墨烯 (GO) 对毛桦的影响微克隆。发现，纳米材料在培养建立阶段保护植物免受植物病原体的侵害，并促进枝条存活率提高 25%。1.5和3 μg/L处理组进一步生长刺激，芽长分别增加1.8和1 cm，叶片数分别增加2和1.5倍。15 μg/L 使芽的高度降低了 20%。在繁殖阶段，添加 GO 可提高 20% 的幼苗成活率，并对桦树再生体的生长和叶片数量有刺激作用。幼苗存活率数据及其形态分析结果与生化评估结果吻合良好，即GO组的光合和过氧化氢酶活性均超过对照组2和1。5次，分别。同时，组织学分析显示 GO 治疗组的气孔数量减少。在生根阶段，在生长培养基中添加 1.5 μg/L GO，与对照相比，带根的植株数量增加了 10%。