Tumor micro-angiogenesis and lymphangiogenesis are effective prognostic predictors in many solid malignancies. However, its role on Xp11.2 translocation RCC has not been fully elucidated. Herein, we purposed to explore the correlation between quantitative parameters of tumor-related micro-angiogenesis or lymphangiogenesis and the prognosis of Xp11.2 translocation renal cell carcinoma (Xp11.2 translocation RCC). Tissue samples were obtained from 34 Xp11.2 translocation RCC and 77 clear cell renal cell carcinoma (ccRCC) between January 2007 and December 2018. Micro-angiogenesis was detected using CD34 antibody and quantified with microvessel density (MVD) and microvessel area (MVA), while the lymphangiogenesis in RCC was immunostained with D2–40 antibody and assessed using lymphatic vessel density (LVD) and lymphatic vessel area (LVA). The Kaplan-Meier method of survival analysis was used to estimate prognosis, and both univariate and multivariate analysis was performing using the Cox proportional hazards. The MVD and MVA of Xp11.2 translocation RCC in two detected areas (intratumoral and peritumoral area) were not significantly different from that of ccRCC (all P > 0.05). Notably, D2–40-positive lymphatic vessels of Xp11.2 translocation RCC were highly detected in the peritumoral area compared to the intratumoral area. Interestingly, the peritumoral LVD and LVA of Xp11.2 translocation RCC were higher than that of ccRCC (all P < 0.05). Furthermore, both intratumoral MVD or MVA and peritumoral LVD or LVA were significantly associated with pT stage, pN stage, cM stage, AJCC stage, and WHO/ISUP grade (all P < 0.05). Univariate analysis of Cancer-specific survival (CSS) revealed that CSS was substantially longer in patients with low intratumoral MVD or MVA than in patients with high intratumoral MVD or MVA (P = 0.005 and P = 0.001, respectively). Lastly, the Cox proportional hazards model in CSS demonstrated that both intratumoral MVD or MVA and peritumoral LVD or LVA were not independent prognostic parameters (all P > 0.05). This study outlines that Xp11.2 translocation RCC is a highly vascularized solid RCC, characterized by rich lymph vessels in the peritumoral area. Quantitative parameters of micro-angiogenesis and lymphangiogenesis could not be considered as novel prognostic factors for patients with xp11.2 translocation RCC.

中文翻译：

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肿瘤相关的微血管生成和淋巴管生成是否被视为Xp11.2易位肾细胞癌患者的新预后因素？

在许多实体恶性肿瘤中，肿瘤微血管生成和淋巴管生成是有效的预后指标。但是，其在Xp11.2易位RCC中的作用尚未完全阐明。本文中，我们旨在探讨肿瘤相关的微血管生成或淋巴管生成的定量参数与Xp11.2易位肾细胞癌（Xp11.2易位RCC）的预后之间的相关性。在2007年1月至2018年12月之间，从34个Xp11.2易位RCC和77个透明细胞肾细胞癌（ccRCC）中获取组织样品。使用CD34抗体检测微血管生成，并用微血管密度（MVD）和微血管面积（MVA）进行定量，而RCC中的淋巴管生成是用D2–40抗体免疫染色的，并使用淋巴管密度（LVD）和淋巴管面积（LVA）进行评估。Kaplan-Meier生存分析方法用于评估预后，单变量和多变量分析均使用Cox比例风险进行。Xp11.2易位RCC的MVD和MVA在两个检测到的区域（肿瘤内和肿瘤周围区域）与ccRCC差异不显着（所有P> 0.05）。值得注意的是，与肿瘤内区域相比，在肿瘤周围区域高度检测到Xp11.2易位RCC的D2–40阳性淋巴管。有趣的是，Xp11.2易位RCC的肿瘤周围LVD和LVA均高于ccRCC（所有P <0.05）。此外，肿瘤内MVD或MVA和肿瘤周围LVD或LVA均与pT分期，pN分期，cM分期，AJCC分期和WHO / ISUP等级显着相关（均P <0.05）。对癌症特异性生存率（CSS）的单因素分析表明，肿瘤内MVD或MVA低的患者的CSS明显长于肿瘤内MVD或MVA较高的患者（分别为P = 0.005和P = 0.001）。最后，CSS中的Cox比例风险模型表明，肿瘤内MVD或MVA以及肿瘤周围LVD或LVA都不是独立的预后参数（所有P> 0.05）。这项研究概述Xp11.2易位RCC是高度血管化的固体RCC，其特征是肿瘤周围区域的淋巴管丰富。微血管生成和淋巴管生成的定量参数不能被视为xp11.2易位RCC患者的新预后因素。