Abstract

A new Cu(II) coordination polymer (CP), [Cu(1,3-BDC)Dptztz]·DMF (DMF = dimethylformamide) was synthesized by reaction of the Cu(NO₃)₂·3H₂O with the 2,5-di(4-pyridyl)thiazolo[5,4-d]thiazole (Dptztz) in the presence of the isophthalic acid (1,3-H₂BDC). The as-prepared complex 1 has been structurally studied via single crystal X-ray diffraction and further characterized by elemental analysis, IR spectroscopy, powder X-ray diffraction, and thermogravimetric analysis. Furthermore, after the construction of the intracerebral hemorrhage (ICH) animal model with type IV collagenase. Then, the protective activity of the compound on ICH animal model was evaluated and the mechanism was evaluated. First, the ROS level in the vascular endothelial cells was evaluated by measuring the relative expression of the ROS related genes. Then, the inflammatory responses in the vascular endothelial cells were measured with western blot.

摘要

通过Cu(NO ₃ ) ₂ ·3H ₂ O与2， 5-二(4-吡啶基)噻唑并[5,4-d]噻唑(Dptztz)在间苯二甲酸(1,3-H ₂ BDC)存在下。制备的复合物1已通过单晶 X 射线衍射进行结构研究，并通过元素分析、红外光谱、粉末 X 射线衍射和热重分析进一步表征。此外，在用 IV 型胶原酶构建脑出血 (ICH) 动物模型后。然后，评价了该化合物对ICH动物模型的保护活性，并对其机制进行了评价。首先，通过测量ROS相关基因的相对表达来评估血管内皮细胞中的ROS水平。然后，用蛋白质印迹测量血管内皮细胞中的炎症反应。